Plant Cell-2012-Ln Is a Key Regulator of Leaflet Shape and Number of Seeds per Pod in Soybean

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1、ThePlantCell,Vol.24:4807–4818,December2012,www.plantcell.orgã2012AmericanSocietyofPlantBiologists.Allrightsreserved.RESEARCHARTICLESLnIsaKeyRegulatorofLeafletShapeandNumberofSeedsperPodinSoybeanWNamheeJeong,aSuJeoungSuh,aMin-HeeKim,aSeukkiLee,bJung-KyungMoon,bHongSigKim,candSoon-ChunJ

2、eonga,1aBio-EvaluationCenter,KoreaResearchInstituteofBioscienceandBiotechnology,Cheongwon,Chungbuk363-883,RepublicofKoreabNationalInstituteofCropScience,RuralDevelopmentAdministration,Suwon441-857,RepublicofKoreacDepartmentofCropScience,ChungbukNationalUniversity,Cheongju361-763,Repu

3、blicofKoreaNarrowleafletsoybean(Glycinemax)varietiestendtohavemoreseedsperpodthanbroadleafletvarieties.Narrowleafletinsoybeanisconferredbyasinglerecessivegene,ln.Here,weshowthatthetransitionfrombroad(Ln)tonarrowleaflet(ln)isassociatedwithanaminoacidsubstitutionintheEARmotifencodedbyagene

4、(designatedGm-JAGGED1)homologoustoArabidopsisJAGGED(JAG)thatregulateslateralorgandevelopmentandthevariantexertsapleiotropiceffectonfruitpatterning.Thegenomicregionthatregulatesboththetraitswasmappedtoa12.6-kbregioncontainingonlyonegene,Gm-JAG1.IntroducingtheGm-JAG1alleleintoaloss-of-

5、functionArabidopsisjaggedmutantpartiallyrestoredthewild-typeJAGphenotypes,includingleafshape,floweropening,andfruitshape,buttheGm-jag1(ln)andEAR-deletedGm-JAG1allelesinthejaggedmutantdidnotresultinanapparentphenotypicchange.Theseobservationsindicatethatdespitesomedegreeoffunctionalcha

6、ngeofGm-JAG1duetothedivergencefromArabidopsisJAG,Gm-JAG1complementedthefunctionsofJAGinArabidopsisthaliana.However,theGm-JAG1homoeolog,Gm-JAG2,appearstobesub-orneofunctionalized,asrevealedbythedifferentialexpressionofthetwogenesinmultipleplanttissues,acomplementationtest,andanallelic

7、analysisatbothloci.INTRODUCTIONprimarilydeterminedbythenumberofovulesperplacentaaswellasthefrequencyofembryonicabortions.SoybeanovariesLeavesandflowersdevelopcontinuouslyattheflanksofthecontainfromonetofourovules,indicatingthattheNSPPisshootapicalmeristeminfloweringplants.Asinglemutatio

8、ndetermineda