低温对兔血液流变性影响实验的研究

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1、InfluenceofHypothermiaonthePropertyofHemorheologyofRabbitsAbstract:Objective:TostudytheinfluenceofhypothermiaonthehemorheologyofrabbitsandtheeffectsofShenfuInjectiontothehyperviscosityinthemodelofhypothermiarabbits.Methods:40rabbitsweredividedrandomlyi

2、nto4groups—controlgroup,hypothermia(bodytemperature,Tb=25±1℃)groupandtwoShenfugroups.The3%pentobarbital-anesthetizedrabbitswereventilatedafterintubation.ThenShenFuInjectionand0.9%normalsalinewereinjectedintotreatedanimalsandcontrolrespectively.Allrabbi

3、tswereinjectedfentanylandarduanbeforehypothermia.Exceptforcontrolgroup,allrabbits’Tbwereloweredfrom37℃to25±1℃andmaintained3hoursbysurfacecooling.Theirmeanarterialpressure（MAP）andTbhadbeenmonitoredduringtheallexperiment.Bloodsamples(2.5ml)weretakenimmed

4、iatelybeforehypothermia,Tbloweringat25℃,startingwarmingandwarmingend.Allrheologicalassayswerecarriedoutimmediatelyatroomtemperature(22±1℃).Results:wehavedevelopedananimalmodelinwhichstablehypothermiacouldbeestablishedwithoutthecardiacbypass（CPB）.Thismo

5、delthusofferstheinvestigationofvariousrheologicalassaysduringprolongedhypothermia.TheMAPofallrabbitsdecreasedsignificantlyafteranesthesia.TheMAPofthecontrolrabbits3treatedwithsalineintravenouslyincreasedgraduallywithtimewhilethatofthehypothermiaandther

6、abbitstreatedwithShenfudecreasedcontinuouslyintheprocessofhypothermia.TheMAPofthelastgroupanimalincreasedgraduallyafterwarming.TheMAPofthehypothermiarabbitsishigherthanthatofthetreatedwithShenfurabbitswhenTbhadreached25°C,startingwarmingandwarmingend(P

7、<0.05).TheMAPchangebetweentwogroupsofrabbitstreatedwithShenFudidnotmakeanysignificantdifference.Thehemorheologyparameterswithgreatchangehadn’tbeenobservedinthecontrolgroup.Inthehypothermiagroup,theviscosityofbloodincreasedprogressivelyto150%ofthatat36℃

8、whenbodytemperaturewasloweredto25℃.ButthatofShenFu-treatedrabbitswasloweredattheirTbhadreached25℃(P<0.05).Atwarmingend,theviscosityofbloodofallanimalsdecreasedtonormal.Theplasmaviscosityof4grouprabbits’hadn’tbeenobservedwithsignificantl