长江华溪蟹线粒体基因组与其种群遗传结构分析

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1、茎茎塑塞—-——-—————————__--_-_____●-_●●————————————_—_———————————___--_____—————————一一ThemitochondrialgenomeandpopulationgeneticstructureofChinesefreshwatercrabSinopotamonyangtsekienseLDXiuling(InstituteofGeneticResources，CollegeoflifeSciences，NanjingNormalUnive

2、rsity,Nanjing210097)AbstractFreshwatercrabsalespecialtaxainBrachyurancrabswhichcompletelyliveinthefreshwater．SinopotamonyangtsekienseisanendemicspeciesofChina．WedeterminedthemitochondrialDNAsequenceofS．yangtsekienseanddiscussedthephylogeneticstatusoffreshwa

3、tercrabsinBrachyuranaccordingtogenearrangementfeaturesandsequencedatasets．Wealsosequencedre百onsofmitochondrialCOlandND5genesinordertoaddressthegeneticstructureof墨yangtsekiensepopulations．1。ThemitochondrialgenomeS．yangtsekienseandphylogenticstudiesofDecapoda

4、Wedeterminedthecomplete1l，405bpmitochondrialDNAsequenceof&yangtsekiense。Itcontains1proteincodinggenes(PCGs)and12tRNAs，typicalofmetazoanmitochondrialgenomes．WithrespecttOtheancestralmtgeneorder,therewasonlyonetRNA(trnH)translocatedoutofthenad5刊4genejunctioni

5、ntothe帆d3qtnd5genejunction．justastwoPortunoidsandoneXanthoid．Aduplication／randomlossmodelofageneblock正n口d5·HWasproposedtoaccountforthetranslocation．Basedontwodatasetsofprotein—codinggenes(4PCGs：coxl一3，cob；1lPCGs：exceptnadlandnad2whichwereunsequenced)，Decapo

6、daconsistedoftWOsubordersofDendrobranchiataandPleocyemata．TIlegenearrangementfeaturesatnad3--nad5andnad5-nad4junctionscontainusefulinformationforphylogeneticinferenceoftheadvancedgroupswithintheDecapoda．Accordingtoeithersequencedatasetsorgenearrangementfeat

7、uresatbothgenejunctions，Brachyurancrabsw_M'emonophyleticanddivergentbetWeenHeterotremataandThoraeotremata．Potamoidearepresentedby曼yangtsekiensewereclosetoPortunoideaandXanthoide&2．Geneticstructureof＆yangtsekiensepopulationsinferredfrommltochondrtaiDNAsequen

8、cesThisstudyusedmoleculardata犯OIandNDS)toexploregenetics姐lctureof＆yangtsekiense．Eightyindividualswereobtainedrepresenting12localpolulations．Alignmentofallsequencesrevealed29and35haplotypesforCOIandND5．