烟草花叶病毒和番茄花叶病毒在烟草上致病性差异的分子机理分析

烟草花叶病毒和番茄花叶病毒在烟草上致病性差异的分子机理分析

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时间:2019-06-25

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1、AbstractTobaccomosaicvirusBisolate(TMV—B)andTomatomosaicvirusS1isolate(ToMV-S1)istwohighlyrelatedvirusesbelongingtoTobamoviruswithsimilargenomicorganizationandfunction.Interestingly,theycausedistinctsymptomsincommonexperimentalhosts.TMVcansystemicallyinfectNicotianatabac

2、um。A,tabacumCV.BurleyandⅣtabacumCV.Huangmiaoyuwithmosaicsymptom,whileToMViSlocalizedwithininoculatedleafwithnecrotic1esions.BothTMVandToMVinducedhypersensitiveresponse(HR)andproducenecroticlesionsinNglutinosa,^itabacumCV.XanthifNN),ⅣtabacumCV.SamsumfNN),andⅣedwardsonii,h

3、oweverTMVinducelesionsmorequicklyandthesizeoflesionsarebiggerthanthatofToMV.Themainpurposeofthispaperistoexplainthephenomenonmentionedaboveandtoinvestigatethefunctioncomplementationofmovementproteins(MPs)betweenTMVandToMV.EmployingoverlapextensionPCRtechnique,theMPgenein

4、TMV-BinfectiousclonepGEBl7WaSsubstitutedwithToMVMPgene.Toconfirmthegenereplacement,twopairsofprimersspecificforTMVandToMVMPgeneweredesignedandusedtoidentifytherecombinantclonepT/OMP.TheinsertedgeneandfusionregionsequenceWaSconfirmedbysequencing./nvitrotranscriptsofpT/OMP

5、containinfectivityaspGEBl7andpmgenyvirusesCaninteractspecificallywithTMVpolyantiserainWesternblotaSsay.TodefinetheintemhangeabilitybetweentheMPfunctionofTMVandToMV,studiesonhostrange,abilityofsystemicallyinfection,phenotypeoflocalinfectionandthesalicylicacid(SA)levelindu

6、cedbyTMVandT/OMPwereconducted.TheresultsshowT/OMPandTMVgetthesanlehostrange.BothoftheminfectⅣtabacumsystemically,andinducesimilarmosaicsymptom.ThecapacityofsystemicallyinfectionWaSdeterminedbyTAS-ELISAonSamsum(nn),Xanthi(nn),andtomato,nosignificantdifferenceWaSfound.Soth

7、eMPgeneofToMVcaltcomplementthefunctionofMPgeneofTMVinsystemicinfectedhostsandMPgeneisvinottheviralfactordeterminingthedifferentphenotypeofTMVandToMVinⅣtabacum.InChenopodiumquinoaandCamaranticolor.TMVandT/OMPinducesimilarlesions.InⅣglutinosa,ⅣtabacumCV.Xanthi(NN),Ⅳtabacum

8、CV.Samsum(NN),and^】:edwardsonii,thelesionsizeandlesionexternalbrownring,inducedbyT/OMParesmallerthantha

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