烟草马铃薯Y病毒（PVY）病株与温度对烟蚜生长发育的影响

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1、Abstract朋弦Ⅱspe飓fc口P(Sulzer)，whichalsonamedG陀enpeachaphid，isoneofthemostimportantpestsontobacc0，anditisthemostmaininsectVectorofPotatoⅥ11lsY(PVY)，whichcantmsmitPVYtotobacc0．AccordingtostudyandinVestigationsoVertheIastfewyearS，theha珊ofPVYontobaccoisincreasingyea卜by—year．Tlhepresentstudieswereto

2、isolateandidentifytobacc0PVYinchongqing，andinVestigatetheabilityof^矽z比spe，5fc口P(sulzer)totransmit。tobacc0PV)．’nleeffectoftobaccoPVY如dtempefatureonpopulationandlife—tableparamete硌of胍pP坶fc口e(Sulzer)wasalsoiIlVestigated．Themailll弓sultsareasfbUows：lTheisolationandidenti6cationofPVYwasisolatedbyus

3、ingindicatingplant(?VzcDf妇，l口s“，纷s口m)andnecrosisspottedhost(C讫朗opDdz溯tn小口，口比如cDzcl，．)，andwasidentifiedbiologyand∞rologybyusingthegeneticlineageofdi俄fentialhoStandindirectEUSA．Di虢rentsymptomswe咒ob∞Ⅳedindi疵renthosts．1ksystemicmosaicsymptomwasfoundiny85，y87，ⅣE∞砌以口f口妇cIl，Ⅳ￡c硼．口搬g缸砌口阳弛dⅣ配D矗矗厅口s聊行s

4、口m；Necrosisspotwasob∞rvedinC强删叩Ddi姗口朋坦r口“f】．cD肠r．Thesymptomofillfec“伽w於nolfoundinQ豫础，口M砂口幻船．TheOD0fVinIssamplew嬲over0．5，andtheratio0fVilllssampletonegative∞ntrolwasmorethan2．ThatmeanstheVimsisPVY．2TheabilityoftransmittingtobaccoPVYby』蚍比sp甜芍fc口P(Sulzer)ThemonaIitymethodofthesourceplantswascond

5、uctedtoinVestigatethePVYobtainingtime，spreadingtime，andspreadingnumbersande￡fectofVirusbytheaphids．11leresunsshowedthatVimscanbepickeduporspreadbyaphidwithinO．5minutes，theincidenceofadiSea∞obViouslyincreaSedwithincreasingOftimeofpickingupandspreadingVimswithinthreeminutes．Theincidence0fadisea

6、SeiIlcreasedslowlyformorethanthreeminutes．1’ransmissionofthePVYwasachieVedbyoneadultaphid．TheincidenceofadiseaseincreasedobViouslywithincreasingofaphidnumberfornomorcthanthreeaphidsperplant；butaslowincreaseOftheincidencewasfoundatthenumberofaphidsperplantoverthree．Allofthenymphae(2nd．3rd)，the

7、apterousandthealatatecantransmitPVY，andtheabil“yoftransmittingPVYwasintheorderofalatate>apterous>nymphae(2”．3一)．3DeVelopmentandreproductionof^么pP坶i伽P(Sulzer)feedingontobaccoinfectedPVYatdifferenttemperatureThe苎rowlhandreproductjonof^fpPr’?(’口