复合植物源抗应激剂的研制与在中华鳖养殖上的应用分析

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1、ABSTRACTDEVELOPMENToFCOMPOUNDBoTANICALANTI．STRESAGENTANDITSAPPLICATIONINAQUACUI』rUREOF豫加ⅣEY＆阶理孙17=钳SStressisasummationofnon·specificresponsesgeneratedbyexternalorinternalextraordinarystimulitobiota．StressreSponseofaquaticanimalsismainlygeneratedbydeteriorationofaquaticenvironmentandpooruseofdrugs

2、orfeeds．Whenaquaticanimalsareinastressstate，theabilitiesofanabolicreduce，catabolismincrease，andtheimmunefunctionsabate，thusgrowthandreproductionfileinhibited，diseasesoutbreakeventually．Inrecentyears，chinaturtle(砌nyxsinensis)inbreedingoftenexperiencesstressresponsescausedbyaquaticenvironment,feeda

3、ndotherinput，leadingtothedeclinationofthequalityandquantityofsuchafamous-brandaquaticproduct．So，tofindasafe，effective，environmentally衔endlyanti·stresstechniqueOrmethodisveryimportant．Inthisexperiment,weselectedeffectiveherbalornaBlralplantextractsasthecorematerial，gonewiththesupplement,suchasorga

4、nicacidsandvitamins，todevelopacompoundbotanicalanti—stressagent．Theproductcouldpreventorrelievestresssyndrome，andhadaveryimportantroleinanti—oxidant,anti-stress，immunityenhancing，growthpromotingandSOon,bysplashingtowateroraddingtofeed．Weselectedrhubarbanthraquinones，chlomgenicacidandbaicalinfroma

5、varietyofherbsandplantextracts，supplementedbycitricacid,vitaminCandvitaminE，anddeterminedthepercentageofeachcomponentinthecompoundbotanicalanti·stressagent,theratioWasthatrhubarbanthraquinones：chlorogenicacid：baicalin：citricacid：vitaminC：vitaminE=1：1：1：1：2：1．Inpriortodeterminatethisratio，wecompar

6、edthesafety,anti·oxidanteffect,i／llrnunefunctionsandgrowthbetweensinglecomponentandcomplex．Theagentcouldbesplashedtowaterandaddedtofeeds，allthetwowayscouldsignificantlyincreasetheanti-stressability．Inthesplashedway，therationalconcentrationwas0．015～0．020mg·L．1，andintheaddedway，thereasonableamountf

7、eedwas1．O~2．0％0．Resultsshowedthat,allthecomponent(rhubarbanthraquinones，chlorogenicacid,baicalin,citricacid，vitaminCandvitaminE)andcomplexhadnoacutetoxicitytochinaturtle．Theexperimentlastedfor30daysandheldinwatertemper