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ID:39118882
大小:2.11 MB
页数:42页
时间:2019-06-25
《大鼠尾状核出血后感觉皮质神经通路的变化与GFAP的表达》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ABSTRACTObjectiveToinvestigatethechangesinneuralpathwayofprimarysomatosensoryandtheexpressionofGFAPintheintracerebralhemorrhageinrats.MethodsTosetupananimalmodelofcerebralhemorrhage.Atotalof20experimentalratswererandomlydividedintotwogroupswith10ratsineachgroups:controlgroupandexperimentalgroup.Af
2、luorogold(FG)retrogradetracingtechniqueandNisslwereusedtostudythechangesintheneuralpathwayofprimarysomatosensory.Immunohistochemicalmethodwasusedtoobservetheexpressionofglialfibrillaryacidicprotein(GFAP)andthequantitativechangeofastrocytesincerebralcortex,caudatenucleus,thalamusandbrainstem.Result
3、s1.Controlgroup:Thenervecellsoftheratsweresphericalandtriangle,whichrankedclosely.Nisslbodywasdyedtoroyalblue.Experimentalgroup:Inthecaudatenucleus,Therecanclearlyseeobviouslyhemorrhageregion,wherethenumberofnervecellsweredecreasedmarkedlycomparedwiththecontrolgroup.Thepericaryonwassphericalandell
4、ipse.Nisslbodywasdyedtolightblue.2.Controlgroup:ThedistributionofFG—labeledneuronswasobservedunderfluorescentmicroscope.ItwasfoundthattheFG-labeledNeuronsweremainlydistributedintheareaofmotorcodex(M1),somatosensorycortex(S1、S29insularcodex(GI,DI,AID,AIVAIP),basal5nucleus(Lss,LAcbsh,LGP,CeL,CeC,IPA
5、CL,IMG),cerebralganglion(VPM,VPL,PVP’LPMR,IMD,CL,MD,VPPC,Po,PrC).Experimentalgroup:ItwasfoundthattheFG-labeledNeuronsweremainlydistributedintheareaofmotorcortex(M1),somatosensorycortex(S1、S2),insularcortex(GI,DI),basalnucleus(LGP,IPACL),cerebralganglion(VL,VM,VPL,VPM).Thedistributionrangeoftheexpe
6、rimentalgroupsisobviouslysmallerthanthecontrolgroups.3.TheimmunoreactivitiesofGFAPinallthecerebralcortex,caudatenucleus,thalamusandbrainstemofexperimentalgroupweresignificantlyenhancedcomparedwiththecontrolgroupRespectively俨7、ductioninencephalicregionandactivatetheastrocytetoexpressGFAPincerebralcortex,caudatenucleus,thalamusandbrainstem.OurdatesuggestionsthatthechangesinneuralpathwayofprimarysomatosensoryandtheexpressionofGFAP,whichw
7、ductioninencephalicregionandactivatetheastrocytetoexpressGFAPincerebralcortex,caudatenucleus,thalamusandbrainstem.OurdatesuggestionsthatthechangesinneuralpathwayofprimarysomatosensoryandtheexpressionofGFAP,whichw
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