大鼠尾状核出血后感觉皮质神经通路的变化与GFAP的表达

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1、ABSTRACTObjectiveToinvestigatethechangesinneuralpathwayofprimarysomatosensoryandtheexpressionofGFAPintheintracerebralhemorrhageinrats．MethodsTosetupananimalmodelofcerebralhemorrhage．Atotalof20experimentalratswererandomlydividedintotwogroupswith10ratsineachgroups：controlgroupandexperimentalgroup．Af

2、luorogold(FG)retrogradetracingtechniqueandNisslwereusedtostudythechangesintheneuralpathwayofprimarysomatosensory．Immunohistochemicalmethodwasusedtoobservetheexpressionofglialfibrillaryacidicprotein(GFAP)andthequantitativechangeofastrocytesincerebralcortex，caudatenucleus，thalamusandbrainstem．Result

3、s1．Controlgroup：Thenervecellsoftheratsweresphericalandtriangle，whichrankedclosely．Nisslbodywasdyedtoroyalblue．Experimentalgroup：Inthecaudatenucleus，Therecanclearlyseeobviouslyhemorrhageregion，wherethenumberofnervecellsweredecreasedmarkedlycomparedwiththecontrolgroup．Thepericaryonwassphericalandell

4、ipse．Nisslbodywasdyedtolightblue．2．Controlgroup：ThedistributionofFG—labeledneuronswasobservedunderfluorescentmicroscope．ItwasfoundthattheFG-labeledNeuronsweremainlydistributedintheareaofmotorcodex(M1)，somatosensorycortex(S1、S29insularcodex(GI，DI，AID，AIVAIP)，basal5nucleus(Lss，LAcbsh，LGP,CeL，CeC，IPA

5、CL，IMG)，cerebralganglion(VPM，VPL，PVP’LPMR，IMD，CL，MD，VPPC，Po，PrC)．Experimentalgroup：ItwasfoundthattheFG-labeledNeuronsweremainlydistributedintheareaofmotorcortex(M1)，somatosensorycortex(S1、S2)，insularcortex(GI，DI)，basalnucleus(LGP,IPACL)，cerebralganglion(VL，VM，VPL，VPM)．Thedistributionrangeoftheexpe

6、rimentalgroupsisobviouslysmallerthanthecontrolgroups．3．TheimmunoreactivitiesofGFAPinallthecerebralcortex，caudatenucleus，thalamusandbrainstemofexperimentalgroupweresignificantlyenhancedcomparedwiththecontrolgroupRespectively俨

7、ductioninencephalicregionandactivatetheastrocytetoexpressGFAPincerebralcortex，caudatenucleus，thalamusandbrainstem．OurdatesuggestionsthatthechangesinneuralpathwayofprimarysomatosensoryandtheexpressionofGFAP，whichw