KiSS-1基因在人绒毛膜癌细胞株上的表达与调控

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1、华中科技大学周济医学院2006届硕：{：毕业论文TheexpressionandregulationofKiSS一1inhumanchoriocarcinomacellsDepartmentofGynecologyandObstetrics，Tong]ihospital，TongjiMedicalCollege，HuazhongScienceandTechnologyUniversityPostgraduate：SunChenTutor：ProfessorZhongGangAbstract0bjective：Ourpurposewastostudythe

2、expressionofKiSS一1mRNAandproteininhumanchoriocarcinomacelllineBeWoandtheregulatoryeffectofdifferentconcentrationofestrogenandprogesteroneonKiSS·1mRNAandproteininhumanchoriocarcinomacelllineBeWo．Methods：TheBeWocellswereincubatedincommonconditioninvitro，theeffectofdifferentconcentr

3、ationof17·betaestradiolandmedroxyprogesteroneacetate(MPA)onKiSS一1mRNAandproteininhumanchoriocarcinomacelllineBeWoweredemonstratedbyRT-PCRandWesternblot．Results：1．BoththeexpressionofKiSS一1mRNAandproteinwereinBeWocells．3华中科技大学同济医学院2006届硕士毕业论文2．Thereshowsdifferentcharactersontheregu

4、lationofdifferentconcentrationof17-betaestradiolontheexpressionofKiSS一1mRNAandprotein．Lowerconcentrationof17．betaestradiol(10一retool／1，10{mmol／1)increasedtheexpressionofKiSS·1mRNAandprotein，thoughhigherconcentrationof17一betaestradiolhadtheoppositeeffect(p<0．05)，3．Differentconcent

5、rationofmedroxyprogesteroneacetate(MPA)(10～retool／1，106mmol／l，lO'Smmol／1)allincreasedtheexpressionofKiSS．1mRNAandprotein(p．(O．05)，butwiththeconcentrationofmedroxyprogesteroneacetate(MPA)raised，theexpressionofKiSS一1mRNAandproteingraduallydecreased(thedataallhavesignificantstatisti

6、cdifferenceexceptgrade10～mmoi／1and10。Smmoi／I)．ConcIusiOⅡs：1．ThemetastasissuppressorgeneKiSS-1islocatedinhumanchoriocarcinomacelllineBeWo，thatsuggeststhegenemayhasregulativeeffectontheinvasiveabilityoftrophoblastcells．2．Differentconcentrationofestrogenandprogesteronebothhaveregula

7、tiveeffectontheexpressionofKiSS一1mRNAandprotein．3．Thephysio．concentration(10。9retool／1)andtentimesbeyondphysio—concentrationof17-betaestradiolbothup-regulatetheexpressionofKiSS·1mRNAandprotein，thissuggestsasthesanleconditionasearlymetaphaseofpregnancytheinvasionoftrophoblastcells

8、aresuppressedthrotIghtheincreasedexpress