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时间:2019-06-24
《KiSS-1基因在人绒毛膜癌细胞株上的表达与调控》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、华中科技大学周济医学院2006届硕:{:毕业论文TheexpressionandregulationofKiSS一1inhumanchoriocarcinomacellsDepartmentofGynecologyandObstetrics,Tong]ihospital,TongjiMedicalCollege,HuazhongScienceandTechnologyUniversityPostgraduate:SunChenTutor:ProfessorZhongGangAbstract0bjective:Ourpurposewastostudythe
2、expressionofKiSS一1mRNAandproteininhumanchoriocarcinomacelllineBeWoandtheregulatoryeffectofdifferentconcentrationofestrogenandprogesteroneonKiSS·1mRNAandproteininhumanchoriocarcinomacelllineBeWo.Methods:TheBeWocellswereincubatedincommonconditioninvitro,theeffectofdifferentconcentr
3、ationof17·betaestradiolandmedroxyprogesteroneacetate(MPA)onKiSS一1mRNAandproteininhumanchoriocarcinomacelllineBeWoweredemonstratedbyRT-PCRandWesternblot.Results:1.BoththeexpressionofKiSS一1mRNAandproteinwereinBeWocells.3华中科技大学同济医学院2006届硕士毕业论文2.Thereshowsdifferentcharactersontheregu
4、lationofdifferentconcentrationof17-betaestradiolontheexpressionofKiSS一1mRNAandprotein.Lowerconcentrationof17.betaestradiol(10一retool/1,10{mmol/1)increasedtheexpressionofKiSS·1mRNAandprotein,thoughhigherconcentrationof17一betaestradiolhadtheoppositeeffect(p<0.05),3.Differentconcent
5、rationofmedroxyprogesteroneacetate(MPA)(10~retool/1,106mmol/l,lO'Smmol/1)allincreasedtheexpressionofKiSS.1mRNAandprotein(p.(O.05),butwiththeconcentrationofmedroxyprogesteroneacetate(MPA)raised,theexpressionofKiSS一1mRNAandproteingraduallydecreased(thedataallhavesignificantstatisti
6、cdifferenceexceptgrade10~mmoi/1and10。Smmoi/I).ConcIusiOⅡs:1.ThemetastasissuppressorgeneKiSS-1islocatedinhumanchoriocarcinomacelllineBeWo,thatsuggeststhegenemayhasregulativeeffectontheinvasiveabilityoftrophoblastcells.2.Differentconcentrationofestrogenandprogesteronebothhaveregula
7、tiveeffectontheexpressionofKiSS一1mRNAandprotein.3.Thephysio.concentration(10。9retool/1)andtentimesbeyondphysio—concentrationof17-betaestradiolbothup-regulatetheexpressionofKiSS·1mRNAandprotein,thissuggestsasthesanleconditionasearlymetaphaseofpregnancytheinvasionoftrophoblastcells
8、aresuppressedthrotIghtheincreasedexpress
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