lps-stimulated il-10 release from neonatal spinal cord microglia is potentiated by glutamate

《lps-stimulated il-10 release from neonatal spinal cord microglia is potentiated by glutamate》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、Neuroscience175(2011)93–103LIPOPOLYSACCHARIDE-STIMULATEDINTERLEUKIN-10RELEASEFROMNEONATALSPINALCORDMICROGLIAISPOTENTIATEDBYGLUTAMATEaa,ba,cE.L.WERRY,G.J.LIU,M.D.LOVELACE,activatedbyahighconcentrationofLPS.©2011IBRO.aaaPublishedbyElsevierLtd.Allrightsreserved.R.NAGARAJAH,I.B.HICKIEANDM

2、.R.BENNETT*aBrainandMindResearchInstituteandBoschInstitute,TheUniversityKeywords:inflammation,lipopolysaccharide,microglia.ofSydney,NSW,2006,AustraliabLifeSciences,AustralianNuclearScienceandTechnologyOrgani-sation,NewIllawarraRoad,LucasHeights,NSW,2234,AustraliaInterleukin-10(IL-10)is

3、amemberofthe4
-helixbundlecDisciplineofAnatomyandHistology,SchoolofMedicalSciences,familyofcytokinesandhasbroadanti-inflammatoryeffectsTheUniversityofSydney,NSW,2006,Australiaonthecentralnervoussystem.IL-10canreducethemor-phologicalsignsofglialactivation(Wirjatijasaetal.,2002).Associat

4、edwiththis,IL-10hasbeenreportedtodecreaseAbstract—Interleukin-10(IL-10)isacytokinewithimpor-theproductionofpro-inflammatorycytokinessuchastu-tantendogenousandtherapeuticanti-inflammatoryef-fects.Giventhis,itisofinteresttoinvestigatefactorsthatmornecrosisfactor-
,IL-1,andIL-6fromactivat

5、edgliamodulateIL-10levelsinthecentralnervoussystem.IL-10(Ledeboeretal.,2000,2002).Glialactivationandhighisreleasedafterlipopolysaccharide(LPS)stimulationoflevelsofpro-inflammatorycytokinescontributetoanum-microglia.Microgliaalsoexpressfunctionalglutamatere-berofcentralnervoussystempath

6、ologiesincludingneu-ceptorsandininflammatoryconditionsareexposedtoropathicpain(Wieseler-Franketal.,2005)andcellularincreasedlevelsofglutamate.Theaimofthisresearch,toxicityresultingfrominfection(Qianetal.,2006b).Appli-then,istoinvestigatewhetherglutamatecanmodulatecationofIL-10canbebene

7、ficialinsuchconditions,forlipopolysaccharidestimulationofIL-10releasefromneo-exampleintrathecalIL-10genetherapyabolishesneuro-natalratspinalcordmicroglia.Enzyme-linkedimmunosor-pathicpaininducedbychronicconstrictioninjury(Milliganbentassays(ELISAs)wereusedtoquantifyIL-10releasefromcult

8、uredn