玻璃表面修饰的基底材料对细_胞粘附分析

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1、北京化工大学硕士学位论文制备过程中没有引入其他金属杂质，不需要额外的除杂过程，因此这种复合膜在组织工程学中具有一定应用前景。对于MCF-7细胞来说，虽然氧化石墨烯的加入提高了粘附效果，但是对于MCF-7细胞的生长和增殖产生了负面的影响。关键词：细胞粘附，玻璃，3一氨丙基三乙氧基硅烷，壳聚糖，氧化石墨烯AbstractSTUDYONCELLADHESIONWITHTHESUBSTRATESONGLASSABSTRACTBecauseofitsimportantroleinbiologicalfunctions，celladhesionhavebeenappliedwidelyincytology

2、andtissueengineering．Inthiswork，inordertoimprovetheadhesioneffectoftheamineslides，thevapordepositionmethodwasdeveloped．MoreoveLgrapheneoxide—chitosancompositefilmswasmadeandcoveredontheglasshopingtoimprovecelladhesioneffectofthesubstrate．Thecontentoftheresearchworkareasfollows：(1)Inthispart，twodiffe

3、rentdepositionmethodsof3-aminopropyltriethoxysilane(APTES)onglassslideswerecomparedinordertostudytheadhesioneffectofcervicalexfoliatedcellsonsmearslides．Glassslidesweremodifiedbyvapor-phasedeposition(V-D)andliquid-phasedeposition(L-D)，respectively．ThenumbersofcellsadheredontheslidesfunctionalizedbyV

4、-DandL-Dwerecountedandcomparedunderthemicroscope．ThenumberofcellsontheslidesfunctionalizedbyV-DandL—Dwas3457土668and2771土693，respectively．Thedatashowedsignificantdifferencesbetweenthetwomethods(t-test：P

5、hinPrepcytologictest．III(2)Grapheneoxideweredispersedinchitosan／aceticacidsolutions．Grapheneoxide．chitosanfilmswereproduced．Themechanicalpropertiesofcompositefilmsweretestedbynanoindentationmethod．Withtheadditionofasmallamountofgrapheneinchitosan(O．1-2．0wt％)，theelasticmodulusandhardnessofchitosanfil

6、mswereincreased．ThecelladhesionresultshowedthattheHepG2cellandMCF-7cellcanadheretoanddeveloponthegrapheneoxide—chitosancompositefilmsaswellasonpurechitosanfilm，indicatingthatgrapheneoxide—chitosancompositeshavegoodbiocompatibility．Becausethereisnometallicimpurityintheproductionstepsofgrapheneoxide，t

7、hetime·consumingpurificationprocessforremovingmetalnanoparticlesisthusavoidedwhengrapheneoxideisusedtopreparebiomedicalmaterials．Grapheneoxide—chitosanfilmsarepotentialcandidatesasscaffoldmaterialsint