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1、上海交通大学硕士学位论文常用口腔修复材料表面细菌粘附的实验研究姓名：张任秀申请学位级别：硕士专业：口腔临床医学（口腔修复学）指导教师：郑元俐20080509上海交通大学硕士学位论文结论：3种细菌混合培养时，Pg受到强烈的抑制。如要抑制Ua的附着和定植，对于钴铬合金修复体而言，前6个小时是关键期。而对于烤瓷修复体，前12个小时是关键期；如要抑制An的附着和定植，对于钴铬合金修复体而言，前24小时是关键期，而对于烤瓷修复体，前12个小时是关键期。材料的粗糙度与细菌形成集落的数量无明显关联。烤瓷修复体优于钴铬合金修复体。关键词：细菌粘附，混合培养，修复材料，粗糙度
2、上海交通大学硕士学位论文ANANALYSISOFBACTERIUMADHESIONSONTHESURFACEOFRESTORATIVEMATERIALSINVITROABSTRACTCrownsarewidelyusedforthetoothdefect.However,aftertheusewilloccur,onthesurfaceoftherestorativematerials,thebacteriumadhesion,whichmaycausesecondarycariesandgingivitisand,inturn,theperiodontal
3、diseases.Objective:Mutans(Ua),porphynomonasgingivalis(Pg)andactinomycesNaeslundii(An)aresaidtobethe3bacteriathatmaycausetheclonalformation,gingivitisandperiodontitis.Withtheobservationoftheiradhesiononthesurfaceofthedentalrestorativematerialsandenamel,weanalyzedthesequenceofthesead
4、hesionsandoffernowtheoptionsforthepreventionoftheiroccurrence.Methods:ThespecimenmadeofPFManddentalCo-Cralloywerepolishedstepbystep.Thenthey,alongwiththeenamelwereputintothe上海交通大学硕士学位论文liquidBHIculturemediumcontainingUa,PgandAn.After6h,12hand24h,theadhesivemicrobialamountandmicrobi
5、alamountintheculturemediumweredeterminedbycountingtheclonalformationunit.Results:(1)Pgwasfoundneitheronthesurfaceofspecimen,norintheliquidBHIculturemedium.(2)Thesurfaceroughness(Ra)ofPFMandenamelwasbiggerthanthatofCo-Cralloy,butthenumberofadherentbacteriumonthemwassmaller.(3)Theval
6、ueofUa/Anwasalmostthesameamongthe3groupswithin6hours.And12hourslater,themaximalincrementofAnwasseeninthegroupofCo-Cralloyandtheminimalincrementinthegroupofenamelwhile24hourslater,thelargestnumberofUa/AnwasseeninthegroupCo-Cralloy.TherewerenochangesinthegroupsofPFMandenamelduring12a
7、nd24hours.Conclusion:Pgwasinhibitedwhenthesebacteriawerecultivatedtogether.IfwewishtopreventtheadhesionofUa,thefirst6hoursisthecriticalperiodforCo-Cralloy,andthefirst12hoursforPFM.IfwewishtoavoidtheadhesionofAn,thekeyperiodisthefirst24hoursforCo-Cralloyandthefirst12hoursforPFM.Give
8、nthesurfaceroughnessofthem
