资源描述：
《培土清心颗粒对特应性皮炎样动物模型的影响_温晓文》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、•1992•当代医药论丛杂志ddyylc.cn·论著·培土清心颗粒对特应性皮炎样动物模型的影响111222温晓文，赵巍，孟威威，刘俊峰，莫秀梅，陈达灿12（广州中医药大学，广州510405；广东省中医院皮肤科，广州510120）摘要：目的：建立特应性皮炎样小鼠模型，探讨中药培土清心颗粒对特应性皮炎细胞免疫的作用机制。方法：以半抗原2-氟二硝基甲苯（DNFB）诱导C57BL/6小鼠建模，造模第1天开始灌胃给药，连续14d。根据局部皮肤症状与组织病理学进行模型评估，计算耳廓肿胀程度和流式细胞检测脾脏调节性B细胞表达变化。结果：模型组皮肤出
2、现明显炎性改变，耳部肿胀明显，病理提示表皮增厚、真皮炎性细胞浸润。培土清心颗粒炎性反应抑制率++++为82.73%；培土清心颗粒组脾脏CD5B细胞、CD1dCD5CD19B细胞阳性表达率均高于模型组，组间比较差异具有统计学意义（P<0.01）。结论：中药培土清心颗粒能有效治疗特应性皮炎，其治疗作用与抗炎、刺激调节性B细胞增殖有关。关键词：特应性皮炎；培土清心颗粒；动物模型；细胞免疫基金资助：广东省中医药强省建设专项中医优势病种突破项目（No.粤财社[2015]19号），广东省质检局项目（No.粤质监标函2015[588号]），广东省中
3、医药强省科研课题（No.20142066）EffectsofPeituQingxinGranulesonatopicdermatitis-likemice111222WENXiao-wen,ZHAOWei,MENGWei-wei,LIUJun-feng,MOXiu-mei,CHENDa-can12(GuangzhouUniversityofChineseMedicine,Guangzhou510405,China;DepartmentofDermatology,GuangdongHospitalofTraditionalChinese
4、Medicine,Guangzhou510120,China)Abstract:Objective:Toestablishatopicdermatitis-likemousemodelandtoexplorethecellularimmunitymechanismsofPeituQingxin(PTQX)GranulesoftraditionalChinesemedicineonatopicdermatitis.Methods:Atopicdermatitis-likeC57BL/6mousemodelwasinducedby2-4-
5、dinitrotoluene(DNFB),themiceweretreatedwithoraladministration,andthemodelwasevaluatedthoughlocalskinsymptomsandpathology.TheswellingdegreeofmiceearwascalculatedandtheexpressionofsplenicregulatoryBcellswasdetectedbyflowcytometry.Results:Theskinofmiceinmodelgroupshowedobv
6、iouslyinflammatorychanges,earswellingobviously,andthepathologydemonstratedthattheepidermiswasmorethickenandthedermisinflammatorycellwasinfiltrated.Theinflammationinhibitionrateofatopicdermatitiswas82.73%inthePTQXgroup.Theexpressionofspleen++++CD5BcellsandCD1dCD5CD19Bcel
7、lsinthePTQXgroupwerehigherthanthoseoftheothergroups,andthedifferencebetweenthreegroupswerestatisticallysignificant(P<0.01).Conclusion:PeituQingxinGranulescouldeffectivelytreatatopicdermatitis,itsmechanismmayberelatedtoanti-inflammationandstimulatetheproliferationofregul
8、atoryBcells.Keywords:Atopicdermatits;PeituQingxinGranules;Animalmodel;CellularimmunityFunding:TraditionalChine
