omega 磁珠法RNA提取说明书

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1、Mag-BindTotalRNAKitMag-BindTotalRNA96KitTableofContentsIntroduction....................................................................2KitContents.....................................................................3PreparingReagents/StorageandStability....................4Mag-BindTota

2、lRNAKitProtocol(M6930)....................5Mag-BindTotalRNA96KitProtocol(M6731)...............7TroubleshootingGuide.................................................10ManualRevision:November2010Innovationsinnucleicacidisolation1IntroductionTheMag-BindTotalRNAKitandMag-BindTotalRNA96Kital

3、lowrapidandreliableisolationofhigh-qualitytotalcellularRNAfromculturedcellsandtissues.TotalRNAfrom1x106ofculturedcellscanbeprocessedinlessthan1hour.ThesystemcombinestheinnovativeSQRNAIsolationTechnologywithMag-BindTissueRNAtechnologytoallowforanautomatableprotocolsfortissuesamples.SQRNA

4、technologyeliminatepolysaccharides,Proteins,andenzymeinhibitorsfromtissuelysate.PurifiedRNAissuitableforallmajordownstreamapplicationssuchasRT-PCR,restrictiondigestion,andhybridizationtechniques.Sincethiskitusesmagneticbeads,tobindRNAandclearlysatetheprotocolcanbeeasilyadaptedtomostrobo

5、ticliquidhandlinginstruments.IfusingtheMag-BindTotalRNAKitortheMag-BindTotalRNA96Kitforthefirsttime,pleasereadthisbookletinitsentiretytobecomefamiliarwiththeprocedures.Samplesarelysedinaspeciallyformulatedbuffercontainingdetergent.DNAandproteinsareeffectivelyremovedbyaquickprecipitation

6、stepwhichisclearedwithmagneticbeads.Theclearedlysateismixedwithmagneticbeads.Afteradjustthebindingcondition,RNAiscapturedbyMag-Bindparticlesonamagnet.Withtworapidwashstep,RNAiselutedwithDEPC-treatedwater.NewinthisEdition:•Thelatestmanualhasbeenredesignedtoenhancereadabilityandlayout.•Ch

7、angeintheLysisandDNARemovalSteps2KitContentsProductNo.M6930-00M6930-01M6930-02Preparations550200Mag-BindParticlesSolutionR55μL550μL2.2mLBufferRCL2mL20mL80mLBufferPNP600μL6mL25mLMagBindParticlesLC110μL1.1mL4.4mLRWB3mL24mL120mLLPABuffer55μL550μL2.2mLDEPCWater600μL10mL40mLInstruct