根结线虫拮抗菌的分离与鉴定

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1、JournalofPlantBiology4IsolationandIdentificationofAntagonisticbacteriaforMeloidogyneincognitainShangqiuZhifangZuoSchooloflifescience,ShangqiuNormalUniversity,Shangqiu476000,Henan,ChinaJournalofPlantBiology4AbstractBiologicalcontrolbecomesincreasinglyimport

2、antinthepreventionofroot-knotnematodedisease.Screeningroot-knotnematodepesticidesmaybenecessaryfornematodecontrol.WeisolatedtwonewantagonisticbacteriaZ-2-36andZ-7-5fromtwentyeightsoilsamplesbyfirstandsecondscreeningsaccordingtoknockdownratesoftheirculturef

3、iltratesagainstMeloidogyneincognitainstarlarvae(J2).Knockdownrateswere95%and97%,respectively.Thesetwobacterialstrainsalsopromotedrootgrowthandreducedthenumberofrootknots.Throughmorphologicalandmolecularcharacterizations,theisolatedantagonisticstrainsZ-5-54

4、andZ-7-5wereidentifiedasArthrobacterpascensandStaphylococcusspecies.KeywordsNematode·Isolation·Identification·Antagonisticbacteria·Arthrobacterpascens·StaphylococcusZ.Zuo(*)SubtropicalHorticultureResearchInstitute,JejuNationalUniversityJeju690-756,Koreae-m

5、ail:zhifangzuo@gmail.comIntroductionThesoil-borneroot-knotnematodediseasegreatlyaffectstheworldagriculturalproduction(XiaoningYan2003).AccordingtoFAO’sconservativeestimate,nematodesdamaged21kindsofcropscosting$7.7billioneconomiclosseseveryyear(NingXie2005)

6、.Long-termuseofchemicalpesticidesagainsttheroot-knotnematodesbroughtaseriesofsideeffectssuchasdamagetotheecologicalbalance,pollutiontowaterandsoilandtheriseindrugresistance(Mankau.R.1980).Developmentofbiologicalnematodepesticidesshouldbeanimportantdirectio

7、n.Bio-pesticidesnotonlydecreaseharmtotheenvironment,butalsocontributetoenvironmentalconservation.Manyplanthealth-promotingrhizobacteria(PHPR)whichmayproducenematicidesubstanceorchangetherelationshipbetweenrootexcretionandnematodestocontrolnematodeshavebeen

8、discoveredincludingPseudomonasfluorescens ,Bacillussphaericus,Bacillussubtillis,Pseudomonaschlororaphis,butsomeoftherhizobacteriahavenotbeenidentified(MingliangZhu2004).WeisolatedandidentifiedArthrobacterpasc