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时间:2019-06-12
《HIV抗体确证试验不确定结果解析崔海燕1,薛秀娟2,刘》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、HIV抗体确证试验不确定结果的解析崔海燕1,薛秀娟2,刘春华2,张倩1,李明1,李颖1,徐涛1(1.驻马店市疾病预防控制中心,河南驻马店463000;2.河南省疾病预防控制中心性病艾滋病防治研究所,郑州450016)摘要:目的分析艾滋病病毒(HIV)抗体不确定者的检测结果,探讨HIV抗体不确定结果出现的规律和HIV不确定人群蛋白印记试验(WB)带型的变化特征。方法对驻马店市HIV确证实验室2013-2015年报告的55例HIV抗体不确定人群进行跟踪随访,回顾性研究其WB带型及HIV酶联免疫吸附试验(ELISA)的S/CO值特征,观察其最终临
2、床转归过程中的发展和变化。结果2013-2015年驻马店地区共检测HIV抗体不确定者55例。经随访检测,有30例(54.5%)确证为阳性,21例(38.2%)确证阴性,4例(7.3%)失访。WB条带的组合模式共有18种。单次带型分析显示,p24带型出现26次,占总条带数的23.0%;其次是gp160,占总带数的19.5%。HIV筛查试验ELISA的不同S/CO值的样本阳转率差异有统计学意义(c2=16.259,P=0.006)。结论驻马店市近几年发现HIV不确定结果中,env带的出现及HIV筛查试验ELISA的S/CO>6.0预示HIV感染
3、的可能性较大,对HIV感染的判断应结合流行病学史及实验室相关检测手段进行综合诊断。关键词:抗体不确定;蛋白印迹试验带型;酶联免疫吸附试验中图分类号:R512.91;R373.9 文献标志码:A文章编号:1672-5662(2017)00-0000-00ToanalyzetheuncertaintyresultsofHIVantibodywasconfirmedbyWesternblotCuiHaiyan1,XueXiujuan2,LiuChunhua2,ZhangQian1,LiMingLiYing1,XuTao.(1.Centerfor
4、AIDS/STDControlandPrevention,ZhumadianCenterforDiseaseControlandPrevention,Zhumadian463000,China;2.CenterforAIDS/STDControlandPrevention,HenanCenterforDiseaseControlandPrevention)Correspondingauthor:XueXiujuan,Email:boboo2009@126.comSupportedbyBasicandadvancedtechnologyres
5、earchofHenan(162300410123)andMedicalscienceandtechnologyresearchprojectsinHenanProvince(201602311)Abstract:objectiveThroughtheanalysisoftheuncertaintyresultsofHIVantibodydetection,theregularityofHIVantibodyuncertaintyandthevariationofWesternblot(WB)bandpatterninHIVuncertai
6、npopulationwerediscussed.Methods55casesofHIVantibodyuncertainpeoplewerefollowedupinHIV-positivelaboratoriesinZhumadianCityfrom2013to2015.Toobservethedevelopmentandchangesinthefinalclinicaloutcomeofthepeople,thecharacteristicsofS/COofEnzyme-linkedimmunosorbentassay(ELISA)an
7、dWBbandwerestudiedretrospectively.Results55casesofHIVantibodyuncertaintyweredetectedfrom2013to2015inZhumadianarea.Afterthefollowing-up,Thirty(54.5%)patientswereconfirmedtobepositive,21(38.2%)confirmednegative,andfourweremissedfollowup.Thereare18combinationsofWBbands.Single
8、bandanalysisshowedthatp24appeared26times,accountingfor23.0%ofthetotalbands,andgp160accoun
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