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页数:3页
时间:2019-05-26
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1、宝生物工程(大连)有限公司TaKaRaBiotechnology(Dalian)Co.,Ltd.Add:辽宁省大连经济技术开发区东北二街19号邮编:116600No.19Dongbei2ndStreet,DevelopmentZone,Dalian(116600),ChinaTEL:0411-87641685,87641686;FAX:0411-87619946,87621675;E-mail:service@takara.com.cn大连TaKaRa产品问题集IDNA芯片相关制品问题集目录Q-1:使用TaKaRaSp
2、acedCoverGlass时需要注意什么?··················································2Q-2:使用TaKaRa-HubbleSlideGlass时需要注意什么?····················································2Q-3:使用λPolyA+RNA-A时需要注意什么?······································································2Q-
3、4:使用RNAFluorescenceLabelingCoreKit(M-MLVVersion)Ver.2.0时,不能得到足够的信号强度是什么原因?····························································································2Q-5:使用RNAFluorescenceLabelingCoreKit(M-MLVVersion)Ver.2.0时,杂交后背景高是什么原因,如何解决?···················
4、···········································································2Q-6:使用RNAFluorescenceLabelingCoreKit(M-MLVVersion)Ver.2.0时,杂交后背景不均一的原因是什么?··································································································3Q-7:RNATransc
5、riptSureLABELTMCoreKit标记aRNA的收量少,或者荧光标记效率低,结果导致了DNA芯片解析的信号较弱,该如何解决?·························································3Q-8:使用RNATranscriptSureLABELTMCoreKit时,DNA芯片杂交后背景较高,如何解决?················································································
6、·····································31Q-1:使用TaKaRaSpacedCoverGlass时需要注意什么?A-1:使用TaKaRaSpacedCoverGlass时需要注意如下事项:1.使用时不要将CoverGlass上下方向、正反面弄错。2.根据点样面的大小选择使用CoverGlass。使用TaKaRaDNA芯片系列产品时,点样区域只在上框时,请使用CoverGlassS;点样区域在上下框时,请使用CoverGlassLorXL。3.注意不要用手接触CoverGlass的表
7、面。4.为防止探针溶液干燥,请在杂交箱或在保湿箱中进行杂交。Q-2:使用TaKaRa-HubbleSlideGlass时需要注意什么?A-2:使用TaKaRa-HubbleSlideGlass时需要注意如下事项:1.使用时,必须先室温放置至恢复室温后,再将铝膜包装开封后使用。2.SlideGlass的四角中,切角处置于右上方时,其表面为DNA样品的点样面,从上端起15mm内为非点样区,可用于粘贴标签、记录样品名等(见下图)。Q-3:使用λPolyA+RNA-A时需要注意什么?A-3:由于RNA容易分解,应避免反复冻融。
8、可用DEPC处理水稀释成适当浓度后,适量分装保存。使用本制品时应遵从RNA实验操作规程,防止RNA分解。Q-4:使用RNAFluorescenceLabelingCoreKit(M-MLVVersion)Ver.2.0时,不能得到足够的信号强度是什么原因?A-4:由于下列原因,标记产物量少,造成探针荧光强度不足。1.RNA量少、表
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