交联对胶原生物学和物理性能的影响

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1、中国组织工程研究与临床康复第73眷第3助2009—01—15出版JoumalofClinicalRehabilitativeTissueEngmeeringResearchJanuary15,2009Vo1．13,No．3灾震基础实验交联对胶原生物学和物理性能的影lIAr*杨春蓉InfluenceofcrosslinkingonthebiologicalandphysicalpropertiesofcollagenYangChun-rongAbstractBACKGROUND：Water．soluble1-ethyl-3一(3-dimethylaminopropy1)carbodii

2、mide(EDC)andN·hydroxysuccinimide(NHS)areDepartmentofMaterialsSciencenon-toxicandgoodbiocompatiblecrosslinkingagentswhichcantriggercollagentocrosslinkandformamidebond．andEngineering，OBJECTIVE：Toanalysistheinfluenceofcrosslinkingonthebiologicalandphysicalpropertiesofcollagen．FujianUniversityofDES

3、IGN．TIMEANDSE1-rING：AcrosslinkingexperimentofcollagenwasperformedattheLaboratoryinDepartmentofTechnology，FuzhouMaterialsScienceandEngineeringofFujianUniversityofTechnologyfr0mSeptembertoNovember2008．350011，FujianProvince．ChinaMATERIALS：I—typebovinecolagensolutionwasprovidedbyGuangzhouChuang’erC

4、ompany．EDCandNHS，asthecrosslinkingagents，wereprovidedbyShanghaiGillBiochemicalCompanyandShanghaiBo’aoBiologicalTechnologyLimited、rangChunrong'~-．Company，respectively．2-(N-Morpholino)ethanesulfonicacid(MES)，asabufer，wasprovidedbyShanghaiJuyuanBiologicalDoctor,Lecturer,TechnologyLimitedCompany．De

5、partmentofMaterialsScienceMETHODS：Collagensolutionwasfreezedat一80oCfor12hoursinrefrigerator．Afervacuumfreeze-drying24hoursforandEngineering，completedehydrationinfreeze-dryingequipment，collagenmatriceswereobtained．ThecollagenmatriceswereimmersedintoFujianUniversityofdiferentconcentrationofEDC．NH

6、SwasaddedaccordingtoEDC：NHS=4：I．TheMESwasasthebuffertoadjustpHt03．O．Technology，FuzhouThenthesolutionwastreatedasfollow：washedbydeionizedwater，vacuumfreeze-dryingforcompletedehydration．350011，FujianProvince，ChinaMAINOUTCOMEMEASURES：Themicrostructure，shrinkagetemperature，characteristicsofswelling

7、dynamicsandabilityoflambmeo@163．comantidegradationofcollagenbeforeandaftercrosslinkingwereanalyzed．RESULTS：Thestudiesofcollagencrosslinkingshowedthatthefree—NH2functionalgroupofcollagenmoleculeschangedintoSupportedby：theN．Hfunotio