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1、广西科学GuangxiSciences1997,4(1):64～71白头叶猴线粒体ND4基因和3D-环区的序列及其分类地位初探PreliminaryStudiesontheClassificationofTrachypithecusfrancoisibasedonmitochondrialND4andD-LoopDNA刘自民韦毅麻秀珍LiuZiminWeiYiMaXiuzhen(广西区林业厅野生动植物和(中华人民共和国南宁动物自然保护区管理站南宁市七星路530022)检疫所南宁530001)(TheWildlifeandNatureReserveManagingstationofGuangx
2、i(NanningAnimalQuarantineServiceofDepartmentofForestry,QixingRoad,Nanning,Guangxi,530022)P.R.China,Nanning,530001)王文陈永久张亚平WangWenChenYongjiuZhangYaping(中国科学院昆明动物研究所细胞与分子进化开放研究实验室云南昆明650223)(LaboratoryofCellularandMolecularEvolution,KunmingInstituteofZoology,ChineseAcademyofSciences,Kunming,Yunnan,6
3、50223)摘要用PCR(多聚酶链式反应)扩增方法测定白头叶猴、黑叶猴和菲氏叶猴各1只的线粒体DNAND4整个基因(1377bp)和D2环500多bp序列。黑叶猴与菲氏叶猴的ND4基因序列碱基差异数高达123,基于ND4基因的遗传距离值为819%;白头叶猴与黑叶猴ND4基因序列间碱基差异数为25,遗传距离值为118%。在D2环区域,白头叶猴与黑叶猴间的遗传距离为413%,而白头叶猴、黑叶猴两者与菲氏叶猴间的遗传距离则分别高达2614%和2610%。分析结果表明,白头叶猴恐怕还只是黑叶猴的一个亚种。从保护遗传学角度看,白头叶猴应被看作一个进化显著性单元(evolutionarysignific
4、antunits,ESU),在动物的保护行动中应得到充分的重视。关键词白头叶猴ND4序列D2环序列分类地位AbstractMitochodrialND4(1377bp)andDLoopDNA(500bp)sequenceswereemployedforestimatingphylogeneticrelationshipsamongwhiteheadleafmonkey,blackleafmonkey,andphayreileafmonkey.ThevariablesitesandgeneticdistanceofND4sequencesbetweenblackleafmonkeyandpha
5、yrcileafmonkeywere123,and819%respectively,whilethosebe2tweenwhiteheadleafmonkeyandblackleafmonkeywere25,and118%.MitochondrialDLoopDNAwascharacteristicbyevoluatingfaster.BasedonDLoopDNAanalysis,thegenet2icdistancebetweenwhiteheadleafmonkeyandblackleafmonkeywas413%,whilethosebe2tweenwhiteheadmonkeyan
6、dblackleafmonkeywere2614%,and2610%respectively.Sofartheblackleafmonkeyandphayreileafmonkeyhavebeenvalidspecies.Ourstudiesimpliedthatwhiteheadleafmonkeyshouldbeasubspeciesofblackleafmonkey,butitshouldbeaevolu2tionarysignificantunits(ESU),whichshouldbetakenseriouslyingeneticconservation.Keywordswhite
7、headleafmonkey,ND4sequence,DLoopsequence,classification中图法分类号Q9591848109[1]白头叶猴首先被谭邦杰于1955年发现并作为一个种,定名为Trachypithecusleucocephalus。其分[2]类地位至今仍有争议。李致祥和马世来于1980年将1996212219收稿。白头叶猴定证为黑叶猴(Trachypithecusfrancois
