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1、第15卷第3期天津农学院学报Vol.15,No.32008年9月JournalofTianjinAgriculturalUniversitySeptember,2008文章编号:1008-5394(2008)03-0007-04*温度骤降对南美白对虾仔虾抗氧化机能的影响121131,通讯作者孙金辉,徐赟霞,季延滨,王庆奎,赵丛明,陈成勋(1.天津农学院水产科学系天津市水产养殖与生态重点实验室,天津300384;2.大连水产学院,辽宁大连116023;3.天津市立达海水资源开发有限公司,天津300300)摘要:测定温度骤降后南美白对虾仔虾肌肉中过氧化氢酶(CatalaseCAT)、超氧化物歧
2、化酶(Superoxidedismutase,SOD)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)的活性和丙二醛(Malondiadehyde,MDA)的含量,评价温度骤降对南美白对虾仔虾抗氧化机能的影响。结果显示:温度由24℃骤降到21℃时,样品中CAT、SOD、ACP、AKP的活性及MDA的含量分别为0.00479~0.00554kU/gport、0.832~0.908kU/gport、0.00219~0.00241U/gport、0.000659~0.000706U/gport及0.465~0.501mmol/L;温度由24℃骤降到18℃时,样品中CAT、SOD、ACP、AKP的活性
3、及MDA的含量分别为0.00420~0.00554kU/gport、0.791~0.908kU/gport、0.00197~0.00243U/gport、0.000643~0.000706U/gport及0.467~0.844mmol/L。由于温度降低到21℃与对照组的温差不大,故各个指标的变化程度不是很明显;而温度降低到18℃后,各指标呈现出了一定的变化趋势,说明温度骤降对南美白对虾仔虾有一定的影响,是南美白对虾健康与存活的重要因素之一。关键词:温度骤降;南美白对虾;抗氧化酶中图分类号:S917文献标识码:AEffectsofSuddenDropinTemperatureonActivi
4、tiesofAntioxidantEnzymesofLitnpenaeusvannamei12113SUNJin-hui,XUYun-xia,JIYan-bin,WANGQing-kui,ZHAOCong-ming,1,CommunicationAuthorCHENCheng-xun(1.DepartmentofAquaculture,TianjinAgriculturalUniversity,Tianjin300384,China;2.DalianFisheriesUniversity,Dalian116023,LiaoningProvince,China;3.TianjinLidaSe
5、awaterResourceDevelopmentLimitedCompany,Tianjin300300,China)Abstract:TheexperimentstudiedtheindexesofLitnpenaeusvannameiinconqueringthesuddendropintemperature.TheactivitiesofCAT,SOD,AKP,ACPandthecontentofMDAwereanalyzed.TheresultsshowthattheaverageactivitiesofCAT,SOD,ACP,AKPandtheaveragecontentofM
6、DAwere0.00479~0.00554kU/gport,0.832~0.908kU/gport,0.00219~0.00241U/gport,0.000659~0.000706U/gportand0.465~0.501mmol/Lwhenthetemperaturedroppedsuddenlyfrom24℃to21℃;theaverageactivitiesofCAT,SOD,ACP,AKPwere0.00420~0.00554kU/gport,0.791~0.908kU/gport,0.00197~0.00243U/gport,0.000643~0.000706U/gportand
7、0.467~0.844mmol/Lwhenthetemperaturedroppedsuddenlyfrom24℃to18℃.Thedifferencesoftemperaturewerenotremarkablebetweentheexperimentalgroupsandthecontrolgroupwhenthetemperaturedroppedto21℃,sothechangesofeachindexweren