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1、·582·ChineseJournalofReparativeandReconstructiveSurgery,June2007,Vol.21,No.6雪旺细胞在大鼠脱细胞异体神经基膜管中迁移的研究王碧菠戴传昌殷德明金羽青祁佐良董佳生【摘要】目的研究大鼠自体雪旺细胞在脱细胞异体神经基膜管中的迁移情况。方法取SD大鼠坐骨神经20mm,用化学萃取法制备脱细胞神经,行大体观察、HE、抗层黏蛋白(Anti2laminin)染色。另取32只雌性SD大鼠,体重250～300g,切取自体坐骨神经2mm,置于分别长10mm的两段大鼠异体脱细胞坐骨神经基膜管之间,形成22mm长的基膜管2自体神经嵌合体,与同样长
2、度的单纯脱细胞神经基膜管埋入肌间隙中,于5、10、15和20d取材,行HE、S2100免疫组织化学染色。结果制备的脱细胞神经基膜管外观呈半透明;HE染色示基膜管内未见细胞核存在,基膜管部分不连续;Anti2laminin染色示基膜管深褐色。基膜管2自体神经嵌合体于术后各时间点HE染色均可见细胞存在,第15天开始可见S2100阳性雪旺细胞;单纯神经基膜管在各时间点HE染色中均可见细胞存在,未见S2100阳性雪旺细胞。结论大鼠坐骨神经的自体雪旺细胞可迁移至两侧异体脱细胞神经基膜管远端,为嵌合自体神经的异体脱细胞神经基膜管修复长段周围神经缺损提供了理论依据。【关键词】雪旺细胞体内培养脱细胞神经基膜管
3、迁移S2100免疫组织化学染色中图分类号:R318108Q813111文献标识码:AASTUDYONMIGRATIONOFSCHWANNCELLSINACELLULARNERVEALLOGRAFTOFRATöWANGBibo,DAIChuanchang,YINDeming,etal.DepartmentofPlasticandReconstructiveSurgery,ShanghaiNinthPeople'sHospital,JiaotongUniversity,Shanghai,200011,P.R.China.E2mail:bbwang0111@hotmail.comCorrespond
4、ingauthor:DAIChuanchang,E2mail:daichch@yahoo.com【Abstract】ObjectiveTostudythemigrationofSchwanncellsfromthenerveautograftintheacellularnerveallograftoftheratsinvivo.MehtodsThesciaticnerves(20mmlong)oftheSDratswereharvestedandpreparedfortheacellularnervegraftsbythechemicalextraction.Then,theywereobse
5、rvedbythegrossview,HEstaining,andAnti2lamininstaining,respectively.Another32femaleSDratsweighing2502300gwereobtainedforthestudy.A22mm2longnerveautograftwasinterposedbetweenthetwo102mm2longnerveallograftstoforma222mm2longcomposite.Then,thecompositewasplacedinthemusclespace,togetherwithasole222mm2long
6、nerveallograftasacontrol.Theywereharvestedat5,10,15and20days,respectively,andwerethengiventheHEstainingandtheS2100staining.ResultsTheacellularnervegraftwassemitransparentunderthegrossview.HEstainingshowedthatnocellwasobservedwithinthenervegraft.Anti2lamininstainingshowedthatthebasalmembranewaspartia
7、llyinterrupted,withapositiveresult(darkbrown).Allthenervegraftsinboththegroupsexhibitedtheexistenceofthecells.TheS2100positivecellswereobservedfromthe15thdayatthefarendsofthetwoallograftsofthecomposit
