资源描述：
《被动转移法致自身免疫性重症肌无力兔模型的建立_李秀华》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、中国神经精神疾病杂志2009年第35卷第1期7论著被动转移法致自身免疫性重症肌无力兔模型的建立*李秀华程焱杨丽谢炳玓梁浩翟翚王虔摘要目的用重症肌无力患者的免疫球蛋白(IgG)建立兔急性被动转移性实验性自身免疫性重症肌无力动物模型。方法提取重症肌无力患者血中的IgG,并将其腹膜内注入长耳白兔,观察实验兔的临床症状、AChRAb滴度、神经肌肉接头电传导功能和病理形态学的变化。结果实验兔于被动转移后第2天渐出现肌无力症状,第3~5天肌无力加重。实验
2、组血清AChRAb吸光度较空白对照组和健康对照组显著性增高(P<001)。重复神经电刺激实验(repetitivenervestimulation,RNS)动作电位衰减率和单纤维肌电图检查平均连续波间值差异均较空白对照组和健康对照组显著延长(P<001)。实验组微小终板电位的波幅和频率较空白对照组和健康对照组均显著性降低(P<001)。实验组单位面积的神经肌肉接头数较空白对照组和健康对照组显著性减少(P<005)。结论被动转移重症肌无力患者的IgG可以诱导出重症肌无力家兔模型。关
3、键词被动转移法实验性自身免疫性重症肌无力AChRAb重复神经电刺激实验神经肌肉接头数中图分类号R746.1文献标识码APassiveTransferofExperimentalAutoimmuneMyastheniaGravisinRabbitswithIgGofMyastheniagravispatients.LIXiuhua,CHENGYan,YANGLi,XIEBingli,LIANGHao,ZHAIHui,WANGQian.Neurology
4、Department,TianjinMedicalUniversityGeneralHospital,154,AnshanRoad,HepingDistrict,Tianjin,300052.Te:l0531-89268106AbstractObjectiveTocreateexperimentalautoimmunemyastheniagravis(EAMG)rabbitsbypassivetransfertheIgG.MethodsCrudeimmunoglobulinfractio
5、nsofmyastheniagravispatientswerepreparedbyammoniumsulphateprecipitationat33%saturationandpurifiedbychromatographyonDEAESephadexA50.ExperimentalanimalswereinjectedIgGintraperitoneally(.ip)andthephenotypewasgradedin4levels.Antiacetylcholinereceptora
6、ntibodieswereevaluatedusingenzymelinkedimmunosorbentassay(ELISA)andelectrophysiologicalfunctionwasassessedbyrepetitivenervestimulation(RNS),singlefibreelectromyography(SFEMG)andminiatureendplatepotential(MEPP)tests.Histologicalstudieswereevaluatedusi
7、ngimmunohistology.ResultsTheseconddayafterthepassivetransfer,alltherabbitsinexperimentalgroupshowedclinicalsignsoffatigabilityandweaknessandtheweaknessexacerbatedonthethirdday.Clinicalsymptomscouldbeattenuatedbyneostigmine.Antiacetylcholinereceptor
8、antibodiesweresignificantlyhigherinexperimentalgroupthaninthehealthycontrolandcontrolgroups(P<001).At3,5,10Hz,thedecrementofcompoundmuscleactionpotential(CMAP)andthemeanofconsecutivedifference(MCD)ofalltherabbitsweresignificantlyhigherinexp
