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1、云南植物研究2006,28(2):203～207ActaBotanicaYunnanica곘提高狗蔷薇离体培养植株再生频率1213余晓丽,马剑民,李玉英,王世茹(1南阳师范学院生物系,河南南阳473061;2河南师范大学生命科学学院,河南新乡453002;3河南南阳农校园艺系,河南南阳473000)摘要:以狗蔷薇(RosacaninaInermis)为材料,以MS为基本培养基,通过对不同植物生长调节剂的组合,大幅度提高了狗蔷薇离体培养植株再生频率。结果表明,2.0mgꄯL6-BA+0.3mgꄯL2,4-D的组合较为适宜,其不定芽再生率为87%,增殖率为3.0;而CPPU和2
2、,4-D的适宜组合为1.5mgꄯL+0.3mgꄯL,其不定芽再生率高达93%,增殖率为5.0。同时,研究结果显示,以MS+40gꄯL蔗糖+6.0gꄯL琼脂粉+3.5mgꄯLAgNO3+1.5mgꄯLCPPU+0.1mgꄯL2,4-D+0.05mgꄯLGA3作增殖培养基效果最好,不定芽诱导率为89%,增殖率为5.5;利于生根的培养基为1ꄯ4MS+20gꄯL蔗糖+3.5gꄯL琼脂+0.3%活性碳+0.1mgꄯLIBA+0.1mgꄯLNAA,生根率为91%。关键词:狗蔷薇;植株再生;组织培养中图分类号:Q945文献标识码:A文章编号:0253-2700(2006)02-203-0
3、5ImprovingofinvitroPlantRegenerationFrequencyofRosacanina(Rosaceae)Inermis1213YUXiao-Li,MAJian-Min,LIYu-Ying,WANGShi-Ru(1DepartmentofBiology,NanyangNormalUniversity,Nanyang473061,China;2CollegeofLifeScienceofHenanNormalUniversity,Xinxiang453002,China;3DepartmentofHorticulture,NanyangAgricu
4、lturalSchool,Nanyang473000,China)Abstract:AnefficientregenerationsystemofRosacaninaInermiswasdevelopedinthisstudy.Theresultsshowedthattheregenerationfrequencyofadventitiousbudswas87%andthemultiplicationratiowas3.0whensupplementedwith2.0mgꄯL6-BAand0.3mgꄯL2,4-DonMurashigeandSkoog(MS)medium,w
5、hiletheregenerationratiowas93%andthemultiplicationratiowas5.0whentheMSmediumcontained1.5mgꄯLCPPUand0.2mgꄯL2,4-D.Moreover,theresultalsoindicatedthatCPPUwasmoreeffectiveintheinductionofadventitiousbuds.Theoptimalmediumforthemultiplica-tionofshootswasMS+40gꄯLsucrose+6.0gꄯLagar+3.5mgꄯLAgNO3+1.
6、5mgꄯLCPPU+0.1mgꄯL2,4-D+0.05mgꄯLGA3,inwhichthemultiplicationratiowas5.5andtheadventitiousbudinductionfrequencywas89%.Thebestmedi-umforrootingandgrowingwas1ꄯ4MS+20gꄯLsucrose+3.5gꄯLagar+0.3%activatedcarbon+0.1mgꄯLIBA+0.1mgꄯLNAA.Therootingfrequencywas91%.Keywords:Rosacanina;Plantregeneration;T
7、issueculture狗蔷薇(RosacaninaInermis),原产欧洲,根。狗蔷薇作为树月季砧木,在国外已普遍用于落叶灌木,其长势强健,无刺、耐寒,抗性强园林绿化,在中欧、北欧各国、美国近海地区,(梁艳华和彭春生,2004),在多种土壤中易于生应用历史较长(deDood等,1990),而我国对树곘基金项目:南阳师范学院2003年应用与科技开发项目(nytc200318);河南省南阳市2004年科技攻关项目(200404016)收稿日期:2005-07-06,2006-02-28接受发表作者简介:余晓丽(19
