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1、安徽农业科学,JournalofAnhuiAgri.Sci.2009,37(9):3901-3902,3910责任编辑罗芸责任校对况玲玲几种沙棘种子萌发及幼苗生长期淀粉酶活性的变化何士敏,汪建华,郭丽佳(长江师范学院生命科学系,重庆408100)摘要[目的]探索沙棘种子萌发的生理机制。[方法]采用3,5二硝基水杨酸法研究沙棘种子萌发及幼苗生长期淀粉酶活性的变化及温度、pH值、底物浓度对其影响。[结果]不同沙棘种子在萌发过程中的淀粉酶活性不同。在3种沙棘中,阿尔泰新闻的酶活性最高,且一直上升,向阳的酶活性最低,优胜的酶活性随种子的萌发先增加后降低
2、。淀粉酶的活性在pH值为6.8时最高。酶活性随着底物(1%淀粉)浓度的增大而增加,但当底物浓度增加到一定值(6%)时,酶活性趋于平稳。沙棘种子萌发期淀粉酶的最适温度为45,高于或低于最适温度都会影响酶的活性,且温度过高会使酶失活。[结论]该研究为开发利用沙棘资源提供了理论依据。关键词沙棘;种子萌发;幼苗生长;淀粉酶;温度;底物浓度;pH值中图分类号S718.43文献标识码A文章编号0517-6611(2009)09-03901-02VariationofAmylaseActivityintheSeedGerminationandSeedlingGrowthPeriodso
3、fSomeHippophaeSpeciesHEShiminetal(DepartmentofLifeScience,YangtzeNormalUniversity,Chongqing408100)Abstract[Objective]ThepurposewastoexplorethephysiologicalmechanismofseedgerminationofHippophaerhamnoides.[Method]ThevariationofamylaseactivityintheseedgerminationandseedlinggrowthperiodsofH.rhamnoi
4、desandtheinfluencesoftemperature,pHvalueandsubstrateconcn.ontheactivityvariationwereresearchedby3,5dinitrosalicylicacidmethod.[Result]TheamylaseactivitiesinthegerminationprocessesofdifferentH.rhamnoidesseedsweredifferent.Amongthe3hippophaespecies,theamylaseactivitywashighestandalwaysincreasingin
5、AETXWandlowestinXiangyang.TheamylaseactivityinYoushengwasfirstincreasedandthendecreasedastheseedsweregerminating.WhenpHvaluewas6.8,theamylaseactivitywashighest.Asthesubstrate(1%starch)concn.wasincreased,theamylaseactivitywasincreased.Butwhenthesubstrateconcn.wasincreasedtoacertainvalue(6%),theamy
6、laseactivitychangedtobesteady.ThefittesttemperatureforamylaseintheseedgerminationperiodofH.rhamnoideswas45,bothhigherandlowertemperatureswouldinfluenceamylaseactivityandtoohightemperaturewoulddeactivateamylase.[Conclusion]TheresearchsupplytheoreticalfoundationfordevelopingandutilizingH.rhamnoide
7、sresources.KeywordsHippophaerhamnoides;Seedgermination;Seedlinggrowth;Amylase;Temperature;Substrateconcentration;pHvalue沙棘(HippophaerhamnoidesL.)为胡颓子科沙棘属的落叶时期;芽长平均为2cm为第2时期;芽长平均为5cm为第3灌木或是小乔木。近几年三北防护林体系工程一直把
