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1、2010;30(8)南方医科大学学报(JSouthMedUniv)窑1903窑内皮抑素不同给药途径联合阿霉素治疗鼠肝移植瘤王泽新1袁汪森明2袁周琪1袁胡喜钢2袁朱伟良2袁孟辉2袁张积仁2渊1重庆市涪陵中心医院肿瘤科袁重庆408000曰2南方医科大学珠江医院肿瘤中心袁广东广州510282冤摘要院目的研究内皮抑素渊Es冤瘤内与尾静脉两种给药途径联合腹腔注射阿霉素渊Adm冤对小鼠H22肝癌细胞移植瘤血管生成和肿瘤生长的抑制作用遥方法将H22肝癌细胞接种到40只小鼠的背部皮下袁肿瘤直径约1cm时按体重随机分成4组曰对照组院隔日瘤内尧腹腔注射生理盐水曰
2、Es瘤内组院隔日瘤内注射Es+腹腔注射Adm曰Es静脉组院隔日静脉推注Es+腹腔注射Adm曰Adm组院隔日静脉推注生理盐水+腹腔注射Adm遥每3d测量皮下肿瘤直径袁绘制肿瘤生长曲线袁第15天每组处死5只小鼠袁检测血浆VEGF含量尧肿瘤组织微血管密度渊MVD冤袁观察剩余小鼠生存期遥结果Es瘤内组小鼠肿瘤体积大小尧VEGF及MVD表达均小于其他3组渊P<0.05冤袁Es瘤内组生存期与Es静脉组比较均较阿霉素组尧对照组延长渊P<0.05冤袁但两者生存期差异无统计学意义渊P>0.05冤遥结论Es联合Adm在抑制血管生成与肿瘤生长方面袁瘤内应用Es抑瘤
3、效果优于静脉应用袁但前者小鼠生存期的延长较后者无明显优势遥关键词院内皮抑素曰阿霉素曰肝肿瘤曰血管内皮生长因子中图分类号院R735.7文献标识码院A文章编号院1673-4254(2010)08-1903-03EndostatinindifferentadministrationroutescombinedwithadriamycinchemotherapyinthetreatmentoflivercancerxenograftinmiceWANGZe-xin1,WANGSen-ming2,ZHOUQi1,HUXi-gang2,ZHUWei-li
4、ang2,MENGHui2,ZHANGJi-ren21DepartmentofOncology,ChongqingFulingCentralHospital,Chongqing408000,China;2DepartmentofOncology,ZhujiangHospital,SouthernMedicalUniversity,Guangzhou510282,ChinaAbstract:ObjectiveTostudytheantiangiogeneticandtumorinhibitoryeffectsofendostatin(Es)by
5、intratumoralversusintravenousadministrationcombinedwithadriamycin(Adm)fortreatmentoftransplantedtumorinmice.MethodsFortymiceweresubjectedtosubcutaneousimplantationofH22cellsandrandomlydividedinto4groupsbythebodyweightwhenthetumordiameterreached1cm,namelythecontrolgroup(with
6、intratumoralandintravenousinjectionofnormalsaline),Esintratumoralgroup(withintratumoralinjectionEsandintraperitonealAdminjection),Esveingroup(withintravenousEsinjectionandintraperitonealAdminjection),andAdmgroup(withintratumoralsalineinjectionandintraperitonealAdminjection)
7、.Thetumorvolumesandtumorinhibitionrateswerecalculated,andtheexpressionofvascularendothelialgrowthfactor(VEGF)andthemicrovesseldensity(MVD)ofthetumorswereexamined,withthesurvivaltimeofthemicealsoobserved.ResultsThetumorvolumewassmallerinEsintratumoralgroupthanintheothergroup
8、s(P<0.05).TheexpressionofVEGFandMVDinEsintratumoralgroupwassignificantlydecreaseda
