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1、中国矫形外科杂志2005年6月第13卷第12期OrthopJChin,Vol.13,No.12June2005·911··基础研究·冻干脱钙骨表面纳米结构△对细胞行为的影响钱张兆锋沈尊理侯明钟摘要目的:探讨表面有纳米结构的冻干脱钙骨基质(nFDBM)的制备方法及该结构对成骨细胞生物学行为的影响。方法:取新鲜狗趾皮质骨按改良Urist法制备冻干脱钙骨基质(FDBM),经Nd:YAG激光表面处理后,用原子力显微镜、扫描电镜观察其表面形态结构学变化。nFDBM、FDBM分别与狗自体骨髓基质细胞诱导分化来的成骨细胞体外构建材料———细胞复合物并植入该狗脊柱两侧深筋膜

2、袋内,于植入前及术后第4、8周取材行扫描电镜及组织学观察。结果:FDBM经Nd:YAG激光处理后表面形成了规则的纳米级沟槽状三维结构。体外构建材料———细胞复合物后扫描电镜观察nFDBM表面细胞黏附密度及分泌基质量均高于FDBM。术后HE染色、扫描电镜观察见左侧植入物基本纤维化,表面未见明显成骨细胞及基质;右侧植入物原纳米沟槽一侧形成薄层骨样组织,表面为成骨细胞分泌的大量基质所覆盖。结论:Nd:YAG激光可在FDBM表面形成纳米级沟槽状三维结构;该结构有利于成骨细胞的黏附、生长和基质分泌。关键词组织工程;冻干脱钙骨基质;纳米;细胞行为中图分类号R683.2文

3、献标识码A文章编号1005-8478(2005)12-0911-04Effectionofnano2structureonthesurfaceoffreeze2drieddemineralizedbonematrixtoosteoblast2behavior∥QIANJun,ZHANGZhao2feng,SHENZun2li,etal.DepartmentofPlastic,ShanghaiFirstPeopleHospital,Shanghai200080AbstractObjective:Toinvestigatethemethodofhowthefre

4、eze2drieddemineralizedbonematrixwithnanoscaletopogra2phy(nFDBM)wasfabricatedandeffectsofthenano2structureonosteoblast2behavior.Method:Allogenicdogs'phalangealcorti2calbonewasfabricatedintofreeze2drieddemineralizedbone(FDBM)withmodifiedUrist'smethod.FDBMwassubjectedtoNd:YAGlaserirra

5、diationunderspecialconditions.Thesurfacetopographywasidentifiedbyatomicforcemicroscopeandscanninge2lectronmicroscope(SEM).Theosteoblastswereinducedfromautologousbonemarrowstromalcells(BMSCs)andmixedwithnFDBMandFDBMinvitro.Cell2materialconstructswereimplantedintofascialbagsondogs'ba

6、ck.TheresultswereassessedbyhistomorphologicandSEMpreoperativelyandin4th,8thweekspostoperativelysurgery.Result:ThesurfaceofFDBMresultedinwell2definedthree2dimensionalnanoscalegrooves.TheosteoblastsfromBMSCsadheredonnFDBMweremorethanthoseonFD2BMandsecretedmoreextracellularmatrixafter

7、implantedonthescaffold.Theconstructsontheleftofdogs'spinebecamefibrosisin4thand8thweekspostoperatively,whilenewbone2likethinlayerswereobservedonthenanoscalesurfaceoftherightcon2structsbyhistologicalexamination.AbundantosteoblastsandmatrixwereobservedbySEMonthesurfaceoftheconstructs

8、ontherightandnoobviouscell