人脑胶质瘤细胞原代培养实验模型的构建

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1、#744#姜政,等人脑胶质瘤细胞原代培养实验模型的构建×临床研究Ø人脑胶质瘤细胞原代培养实验模型的构建11123姜政,江玉泉,李新钢,李冰,周伟1.山东大学齐鲁医院神经外科,山东济南2500122.临沂市人民医院神经外科,山东临沂2760033.山东省肿瘤医院放疗科,山东济南250117ConstructionofthemodelofprimaryculturedhumangliomacellsJIANGZheng1,JIANGYu-quan1,LIXin-gang1,LIBing2,ZHOUWei31

2、.DepartmentofNeurosurgery,QiluHospitalofShandongUniversity,Jinan250012,P.R.China2.DepartmentofNeurosurgery,LinyiPeople.sHospital,Linyi276003,P.R.China3.DepartmentofRadiationOncology,ShandongTumorHospital,Jinan250117,P.R.China【摘要】目的:探索人脑胶质瘤细胞原代[ABSTRACT]O

3、BJECTIVE:Toexplorethemethodofprimaryhuman培养的方法,提高原代培养的成功率,快速gliomacellsculture,toimprovethesuccessrateofprimaryculture,andto构建人脑胶质瘤细胞体外试验模型。方法:establishanexperimentalmodelforstudyinggliomainvitro.METHODS:分别采用组织块培养法和酶消化法对12例Thegliomacellsfrom12gliomapatie

4、ntswereculturedprimarilybythe人脑胶质瘤细胞进行原代培养,比较两种tissuepiecesculturemethodandenzymaticdigestionculturemethod,and方法的培养效果;通过倒置相差显微镜和theeffectsofculturewerecompared.Themorphologywasinspectedbya激光共聚焦显微镜观察细胞的形态学特invertedphasecontrastmicroscopeandaconfocallasers

5、canningmicro-点;通过生长曲线的测定研究体外培养细scopeandthegrowthcurveswereanalyzed.RESULTS:Theprimaryhu-胞的生长特性。结果:原代人脑胶质瘤细胞mangliomacellsweresuccessfullyculturedandtransferred.Thesuccessful短期培养成功,并可传代。经组织块培养法rateofthetissuepiecesculturemethodwas3313%.Thesuccessfulrateof

6、成功率较低为3313%;经酶消化法短期培theenzymaticdigestionculturemethodwas8313%,whichwaspriorto养成功率为8313%,明显高于组织块培养thetissuepiecesculturemethodsignificantly.Theculturedcellshadthe法。培养成功的细胞状态稳定,增殖活跃,proliferativeabilityandlogarithmicgrowthphase.CONCLUSIONS:The有明显的对数生长期。结论

7、:用酶消化法可humangliomacellsmaybeculturedbytheenzymaticdigestionculture进行人脑胶质瘤短期体外培养,且成功率method,whichhashighersuccessfulrate.Theprimaryculturemodelof较高,适宜建立体外细胞培养模型。humangliomacellscanbeestablishedinvitro.中华肿瘤防治杂志,2006,13(10):744-747ChinJCancerPrevTreat,2006,

8、13(10):744-747【关键词】脑肿瘤/细胞学;神经胶质瘤/细胞学;细胞,培养的;细胞培养/方法;免疫组织化学[KEYWORDS]brainneoplasms/cytology;glioma/cytology;cells,cultured;cellculture/methods;immunohistochemistry【中图分类号】R739.41【文献标识码】A【文章编号】1673-5269(2006)10-0744-04人脑胶质瘤是