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1、安徽农业科学,JournalofAnhuiAgr.iSc.i2010,38(8):3914-3915,3924责任编辑姜丽责任校对况玲玲矮生紫薇的组织培养与再生技术研究1112王闯,刘敏,刘殿红,李中勇(1.聊城职业技术学院,山东聊城252000;2.河北农业大学园艺学院,河北保定071001)摘要[目的]筛选矮生紫薇(Lagerstroemiaindicacv.PetitePinkie)组织培养的最佳培养基,建立矮生紫薇植株再生体系。[方法]将灭过菌的矮生紫薇种子分别接种于7种无菌苗新诱导培养基上,将获得的无菌苗新生芽接种于8种快繁培养基上,当无根苗长至2~4cm时切去
2、基部组织,接种于8种生根培养基中培养。[结果]不同的含有一定浓度激素的培养基中的矮生紫薇种子的发芽率明显高于不含任何激素的培养基。矮生紫薇试管苗适宜的诱导培养基为MS+6-BA1.00mg/L+NAA0.10mg/L;适宜的快繁培养基为MS+6-BA2.00mg/L+KT1.00mg/L+NAA0.01~0.05mg/L;适宜的生根培养基为MS+NAA0.50mg/L。[结论]该研究为木本矮生紫薇的组织培养及遗传转化提供了技术支持。关键词矮生紫薇;培养基;无菌播种;组织培养;再生中图分类号S685.99文献标识码A文章编号0517-6611(2010)08-03914-0
3、2ResearchonTissueCultureandRegenerationTechnologyofShortCrapeMyrtle(Lagerstroemiaindicacv.PetitePinkie)WANGChuangetal(LiaochengVocationalandTechnicalCollege,Liaocheng,Shandong252000)Abstract[Objective]TheresearchamiedtoscreenouttheoptmiummediaforthetissuecultureofLagerstroemiaindicacv.Pet
4、itePinkieandsetupitsplantregenerationsystem.[Method]ThesterilizedLagerstroemiaindicacv.PetitePinkieseedswereinoculatedrespectivelyon7kindsofnductionmediaforinducingasepsisseedling.Theinducingasepsisseedlingwasinoculatedrespectivelyon8kindsoffastpropagatingmedia.Whentheywere2-4cm,therootle
5、ssseedlingswereinoculatedrespectivelyon8kindsofrootingmedia.[Result]ThegerminationrateofLa-gerstroemiaindicacv.PetitePinkieseedsonmediaaddingwithdifferentconcentrationhormoneswasobviouslyhigherthanthatinmediawithoutthehormone.ThesuitablemediumforinducingtheLagerstroemiaindicacv.PetitePink
6、ieseedlingswereMS+6-BA1.00mg/L+NAA0.10mg/L.TheoptmiummediumforproliferationwasMS+6-BA2.00mg/L+KT1.00mg/L+NAA0.01-0.05mg/L.TheoptmiummediumforrootingwasMS+NAA0.50mg/L.[Conclusion]ThestudycanprovidetechnologicalsupportforthetissuecultureandgenetictransformationofLagerstroemiaindicacv.Petite
7、Pinkie.KeywordsShortcrapemyrtle(Lagerstroemiaindicacv.PetitePinkie);Medium;Asepticsowing;Tissueculture;Regeneration矮生紫薇(Lagerstroemiaindicavc.PetitPinkie)属千屈菜菌播种的培养基的激素配比见表1。将处理后的矮生紫薇种科紫薇属,又名矮化百日红,属国外选育的新品种,落叶小灌子分别接种于培养基上,每种培养基接种10瓶,每瓶10粒。木,株高30~60cm,花期在6~10月份。近年来,矮
