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1、2011年第1期植物检疫PLANTQUARANTINEVo.l25No.1中国番木瓜枯萎病病原菌的首次鉴定12213冯岩陈健罗金棠夏恭澍杜洪忠(1.仲恺农业工程学院农学院广东广州510225;2.广州市果树科学研究所;3.中国检验检疫科学研究院)122FirstidentificationonpathogenofpapayawiltdiseaseinChina.FengYan,ChenJian,LuoJintang,Xia13Gongshu,DuHongzhong(1.CollegeofAgriculture,Z
2、hongkaiUniversityofAgricultureandTechnology,Guan-gzhou510225,China;2.GuangzhouInstituteofFruitTreeScience;3.ChineseAcademyofInspectionandQuar-antine)AbstractThepathogensofpapayawiltdiseasewereidentifiedonthebasisofpathogenicitytesting,aswellasmorphologicalcha
3、ractersandmolecularidentification.Isolatesofpossiblepathogenicfungiwereisolatedfromstemsofinfectedpapayaplants.Basesonthepathogenicityexperimen,ttheresultsshowedthattheisolatewasthepathogenofpapayawiltdisease.Themorphologicalidentificationshowedthattheisolate
4、wasconsistentwithFu-sariumsolani.Byusingofconservationandvariabilityoftheinternaltranscribedspacer(ITS)offungiribosomeDNA,theisolatewasamplifiedbyPCRwithuniversalprimersofITS1andITS4andthensequenced.These-quencefromtheaboveisolatewascomparedwithtensequencespu
5、blishedinGenbankdatabaseandphylogenitictreeswereconstructedbasedonITSsequencesdata.Theresultsshowedthatthetestedisolate,GU134886、GQ451337、EU625405、EF534183andGQ376115wereclusteredintothesamecladeandallofthemwereFusari-umsolani.Themolecularidentificationshared
6、thesameresultswithmorphologyidentification.Thisisthefirstre-portthatFusariumsolaniisthepathogenofpapayawiltdiseaseinChina.Keywordspapayawiltdisease;morphologicalobservation;Fusariumsolani;molecularidentification摘要从发病的番木瓜茎基部组织分离培养得到纯分离物,采用形态学和分子生物学方法进行分离物鉴定。经过
7、致病性测定证实,分离物确定为番木瓜枯萎病的病原菌;通过形态学观察表明,与茄腐镰刀菌(Fusariumsolani)形态一致。根据真菌的核糖体DNA(rDNA)内转录间隔区(ITS)的保守性和变异性,采用通用引物ITS1和ITS4进行PCR扩增,然后将PCR产物纯化后直接测序,对测试菌株与10株来自GenBank中的菌株ITS序列进行聚类分析,结果表明,测试菌株与GenBank上注册的茄腐镰刀菌GU134886、GQ451337、EU625405、EF534183和GQ376115聚在同一分枝上,分子鉴定结果与形态
8、学观察结果一致,此为国内首次报道。关键词番木瓜枯萎病;形态学观察;茄腐镰刀菌;分子鉴定中图分类号S432.192[1-2]番木瓜(CaricaPapaya)又名木瓜、万寿果,原途。现代医学研究表明,番木瓜所含的番木瓜产于南美洲,是热带、亚热带地区特有的一种果树,碱具有抗癌活性;而木瓜蛋白酶则有助于消化,可17世纪后期引入我国种植,至今有300多年的栽培用于治疗胃炎和慢性消
