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1、研究论文(Articles)微波辅助提取废次烟叶水溶性糖工艺12222杜林洳,徐翠莲,樊素芳,万郑凯,王喜云1.河南农业大学烟草学院,郑州4500022.河南农业大学理学院,郑州450002摘要为了有效地提取废次烟叶中水溶性糖,进一步对水溶性糖生物活性进行基础和应用开发研究,为废次烟叶水溶性糖的开发利用提供依据,采用连续微波辐射方法进行微波萃取工艺的优化,在单因素试验的基础上,通过正交试验和极差分析考查了提取温度、微波功率、提取时间和料水比对水溶性糖提取率的影响。通过极差分析显示影响因素从大到小依次
2、为:料液比>功率>时间>温度,正交实验的最佳提取条件是:料液比1:30、提取功率400W、提取时间20min、提取温度60℃,提取率为28.54%。与经典法和直接水提法相比,微波辅助提取法可将水溶性糖的提取率分别提高6.39%和6.92%,并具有高效、节能、产率稳定、省时等特点。关键词废次烟叶;水溶性糖;微波提取;正交试验;参数优化中图分类号R917文献标识码A文章编号1000-7857(2010)13-0092-05ExtractionofWater-solubleSaccharidesfromA
3、bandonedTobaccoswithMicrowave-assistedTechnique12222DULinru,XUCuilian,FanSufang,WANZhengkai,WANGXiyun1.CollegeofTobacco,HenanAgriculturalUniversity,Zhengzhou450002,China2.CollegeofSciences,HenanAgriculturalUniversity,Zhengzhou450002,ChinaAbstractInorde
4、rtoextractwater-solublesaccharidesfromdiscardedtobaccoseffectively,andtostudytheirbiologicalactivity,themicrowavetechnologyisusedinextractionofwater-solublepolysaccharidesfromdiscardedtobaccosandtheoptimalprocessofextractionwasobtainedbythemethodofcont
5、inuousmicrowaveradiation.Onthebasisofone-factortests,theeffectsofextractiontemperature,microwavepower,extractiontimeandliquid-to-solidratioonthemicrowaveextractionofwater-solublesaccharideswereanalyzedbytheorthogonalexperimentandtherangeanalysis.Theres
6、ultsofrangeanalysisshowthatthefactorsaffectingextractionrateareinthefollowingorderintheirimportance:ratioofsolidtoliquid>microwavepower>extractiontime>temperature.Theoptimizedconditionsare:1:30oftheratioofsolidtoliquid,400Wofthemicrowavepower,20minofth
7、eextractiontimeand60℃ofthetemperature.Theextractionrateof28.54%canbeobtainedundertheaboveconditions,anincreaseof6.39%and6.92%,respectively,ascomparedwiththeclassicalmethodandthedirectwaterextractionThismethodenjoysadvantagessuchashighefficiency,energy-
8、saving,yieldstabilityandtime-saving.Keywordsdiscardedtobaccos;water-solublesaccharides;microwaveextraction;orthogonaltest;optimization环境[2]。烟草中除含有多种特殊的化学物质如烟碱、烟酸、茄尼0引言醇[3]、果胶外,还含有多种糖类成分,这些成分都是很重要的化中国是烟草种植大国,年产烟叶280万t,仅河南省年产学原料[4]。烟梗中含有的