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1、第29卷第2期中华中医药学刊Vo.l29No.22011年2月CHINESEARCHIVESOFTRADITIONALCHINESEMEDICINEFeb.2011局部和远道电针对根性痛大鼠脊髓背角及背根神经节NOS的表达影响11221程博,屠文展,楼新法,庞玲霞,蒋松鹤(1.温州医学院附属第二医院,浙江温州325027;2.温州医学院基础学院,浙江温州325027)摘要:目的:通过同节段局部和远道电针干预治疗慢性根性痛模型大鼠,观察其对脊髓背角及背根神经节中一氧化氮合酶(NOS)活性变化的影响
2、。方法:采用慢性根性痛大鼠模型,将30只雄性Wistar大鼠随机分为正常对照组(A组)、模型组(B组)、局部电针组(C组,取双侧L4/夹脊0穴)、远端电针组(D组,取双侧/阳陵泉0)。通过NADPH-d组织化学染色,观察电针后脊髓背角及背根神经节NOS表达的变化。结果:患侧脊髓背角及DRG中NADPH-d阳性反应结果一致:B、C、D组较A组均增加明显(P<0.01),其中,B组增加尤其明显,较C、D组差异有显著统计学意义(P<0.01);C组阳性表达较D组降低,差异有统计学意义(P<0.05)。
3、结论:电针的镇痛作用可能部分是通过抑制NOS的活性实现的。同节段局部电针组抑制NOS活性的作用较强。关键词:电针;神经病理性痛;脊髓背角;背根神经节;NOS中图分类号:R245文献标识码:A文章编号:1673-7717(2011)02-0317-04EffectsofElectro-AcupunctureonLocalandDistantCoordinateAcupointsontheExpressionofNOSintheSameSegmentsofSpinalCordDorsalHornan
4、dDRGsintheRatswithChronicRadicularPain11221CHENGBo,TUWen-zhan,LOUXin-fa,PANGLin-xia,JIANGSong-he(1.The2ndAffiliatedHospitalofWenzhouMedicalCollege,Wenzhou325027,Zhejiang,China;2.WenzhouMedicalCollege,Wenzhou325027,Zhejiang,China)Abstrac:tObjective:To
5、observetheexpressionofNOSinthesamesegmentsofspinalcorddorsalhornandDRGsintheratswithchronicradicularpain,andtoinvestigatewhethertherewasdifferentaffectionanddifferentneurochemicalmechanismofelectro-acupuncture(EA)onlocalanddistantcoordinateacupointst
6、oNeuropathicpainrats.Methods:A-fterthemodeloftheratswithsciaticnerveClampingbeingmadesuccessfully,thirtymaleWistarratsweredividedintofourgroupsrandomly,includingnormalcontrolgroup(GroupA),modelgroup(GroupB),localacupointsneedlinggroup(GroupC,chooseEX
7、-B2JiajieachsideofL4),distantacupointsneedlinggroup(GroupD,chooseGB34Yan-glingquaneachside).ThechangesoftheexpressionofNOSinthespinalcorddorsalhornandDRGsoftheratswereob-servedafterstainedwithNADPH-d.Results:Comparedwiththenormalcontrolgroup,theexpre
8、ssionofNOSinthesp-inalcordandDRGsintheothergroupsratswereincreasedsignificantly(P<0.01).Andtheincreasinginthemodelgroupismoresignificantthanthetwoelectro-acupuncturegroups(P<0.01).Therewassignificantdecreaseoftheex-pressionofNOSinGroupCcomparedwithth
