免疫化学 自噬LC3

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1、VirchowsArch(2011)459:315–319DOI10.1007/s00428-011-1104-zORIGINALARTICLEImmunohistochemicallocalizationofautophagosomalmembrane-associatedproteinLC3ingranularcelltumorandschwannomaMasayukiShintakuReceived:16March2011/Revised:26April2011/Accepted:30May2011/Publishedonline:15June2011#TheAuthor(s)2

2、011.ThisarticleispublishedwithopenaccessatSpringerlink.comAbstractGranularcelltumor(GCT)isaneoplasmderivedIntroductionfromSchwanncellor(incasesarisingintheneurohypoph-ysis)pituicyteandischaracterizedbyabundantcytoplasmGranularcelltumor(GCT)isaneoplasmwithdistinctivefilledwithnumerouseosinophilic

3、granules,whichhavehistopathologicalandultrastructuralcharacteristics.Itcom-beenconsideredautophagolysosomesonthebasisoftheirmonlyarisesintheskinandsubcutaneoustissue,variousultrastructure.Toconfirmthattheformationofthesevisceralorgans,especiallythebreastandupperaerodigestivegranulesisrelatedtoan

4、autophagyphenomenon,12casestracts,andintheneurohypophysis[1].RegardingtheofGCT(includingtwocasesofGCToftheneurohypoph-histogenesisofGCT,casesofGCTarisingintheskin,ysis)werestudiedimmunohistochemicallyusingananti-subcutaneoustissue,andvisceralorgansarewidelybelievedbodyagainstLC3(microtubule-asso

5、ciatedprotein1lighttooriginatefromSchwanncells[2–7].Thehistogenesisofchain3,aspecificmarkerofautophagy).AllcasesofGCTGCTintheneurohypophysisremainscontroversial,butshowedgranularimmunoreactivityforLC3inthecyto-derivationfrompituicytehasbeenproposed[8,9].Regard-plasmoftumorcells,indicatingthatthe

6、formationoflessoftheirlocalizationororigin,thehistopathologyofGCTintracytoplasmicgranulesinGCTiscloselyrelatedtoanisquiteuniform.Itconsistsofaproliferationoflargeautophagyphenomenon.ForelucidationoftherelationshippolyhedralcellswithabundantcytoplasmwhichisloadedbetweenGCTandschwannoma,20casesofs

7、chwannomawithnumerouseosinophilicgranules.Althoughthesegran-weresimilarlystudiedusingtheanti-LC3antibody.Ineightuleshavebeenconsideredautophagosomesorautophagoly-of20cases,asmallnumberoftumorcellsshowedgranularsosomesbasedon