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1、282ActaPhysiologicaSinica,June25,2004,56(3):282-287http://www.actaps.com.cnResearchPaperEffectsofplateletactivatingfactoronactionpotentialsandpotassiumchannelsinguinea-pigventricularmyocytesDUYi-Mei1,,TANGMing1,*,LIUChang-Jin1,KEQin-Mei2,LUOHong-Yan1,HUXin-Wu11Depar
2、tmentofPhysiology,TongjiMedicalCollegeofHuazhongUniversityofScienceandTechnology,Wuhan430030,China;2DepartmentofGeriatrics,theUnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,ChinaAbstract:Thisstudywasdesignedtoinvestigatetheef
3、fectsofplateletactivatingfactor(PAF)ontheactionpotentialandpotassiumcurrentsinguinea-pigventricularmyocytes.Wholecellpatchclamptechniqueswereused.With5mmol/LATPinthepipetteelectrode(mimicnormalcondition),1µmol/LPAFincreasedAPDfrom225.8±23.3to352.8±29.8ms(n=5,P<0.05)
4、,decreasedIandItail90K1Kcurrentsfrom-6.1±1.3to-5.6±1.1nA(n=5,P<0.05)at-120mVandfrom173.5±16.7to152.1±11.5pA(P<0.05,n=4)at+30mV,respectively.ButPAFhadnoeffectonIatpotentialswithinthenormalrangeofmembranepotentials(between-90mVand+20mV).K1Inthecontrary,withoutATPinthe
5、pipetteelectrodebywhichIwasactivated(mimicischemiccondition),1µmol/LPAFshortenedK·ATPAPDfrom153±24.6to88.2±19.4ms(n=5,P<0.01).Incubationofmyocyteswith1µmol/Lglibenclamide,ablockerofIcould90K·ATPrestoreprolongationofAPDinducedbyPAF.Inconclusion,inguinea-pigventricula
6、rmyocytes,with5mmol/LATPinthepipettePAFcouldprolongAPDpartlyduetotheinhibitionofI;whilewith0mmol/LATPinthepipette,PAFcouldinduceanactivationofI,KK·ATPhenceadecreaseinAPD.ItissuggestedthatPAFmayamplifytheheterogeneitybetweenischemicandnormalcardiacmyocytesduringische
7、mia/reperfusion,whichmayplayavitalroleinthepathogenesisofthearrhythmiasinducedbyischemia/reperfusion.Keywords:plateletactivatingfactor;cardiacmyocyte;actionpotential;potassiumchannels;patchclamptechnique血小板活化因子对豚鼠心室肌细胞动作电位和钾通道的影响杜以梅1,唐明1,*,刘长金1,柯琴梅2,骆红艳1,胡新武11华中科技大学
8、同济医学院生理系,武汉430030;2华中科技大学同济医学院附属协和医院,武汉430022摘要:应用全细胞膜片钳技术研究了血小板活化因子(plateletactivatingfactor,PAF)对豚鼠心室肌细胞动作电位和钾电流的影响。结果发现,当电极内液ATP浓度为5mmo
