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1、第16卷第1期中国当代儿科杂志Vol.16No.12014年1月ChinJContempPediatrJan.2014doi:10.7499/j.issn.1008-8830.2014.01.007论著·临床研究宫内生长迟缓早产儿T淋巴细胞亚群水平变化彭华保 侯彰华 龙伟 谭若锟 唐李维(南华大学附属郴州市第一人民医院新生儿科,湖南郴州 423000)[摘要] 目的了解宫内生长迟缓(IUGR)早产儿生后T淋巴细胞亚群水平及变化。方法67例早产儿中IUGR早产儿29例,适于胎龄(AGA)早产儿38例;另取
2、健康足月儿20例为对照组。采用流式细胞仪测定各组出生24h内及校正胎龄38周时外周静脉血T淋巴细胞亚群水平;同时测定各时间点外周血白细胞、淋++巴细胞及T淋巴细胞绝对计数。结果24h内IUGR早产儿CD3、CD4百分比低于AGA早产儿和对照组(P<0.05),+++++++IUGR早产儿CD8及CD4/CD8低于对照组(P<0.05),AGA早产儿CD3、CD4及CD4/CD8低于对照组(P<0.05);IUGR早产儿外周血淋巴细胞低于对照组(P<0.05),IUGR、AGA早产儿的T淋巴细胞低于对照组
3、(P<0.05),++IUGR早产儿T淋巴细胞低于AGA早产儿(P<0.05)。校正胎龄38周时,IUGR、AGA早产儿CD3、CD4及+++++++CD4/CD8高于出生24h内(P<0.05),IUGR早产儿CD3、CD4、CD8及CD4/CD8低于AGA早产儿(P<0.05);IUGR与AGA早产儿外周血白细胞、淋巴细胞、T淋巴细胞比较差异无统计学意义(P>0.05)。结论IUGR早产儿T淋巴细胞亚群免疫功能低于AGA早产儿及健康足月儿,并且持续至生后一段时间。[中国当代儿科杂志,2014,16(
4、1):31-34][关键词]宫内生长迟缓;T淋巴细胞亚群;免疫;早产儿ChangesinTlymphocytesubsetsinpreterminfantswithintrauterinegrowthretardationPENGHua-Bao,HOUZhang-Hua,LONGWei,TANRuo-Kun,TANGLi-Wei.DepartmentofNeonatology,FirstPeople’sHospitalofChenzhouAffiliatedtoTheUniversityofSouthC
5、hina,Chenzhou,Hunan423000,China(Email:by_phb@sina.com)Abstract:ObjectiveTostudychangesinTlymphocytesubsetsinpreterminfantswithintrauterinegrowthretardation(IUGR).MethodsThestudyenrolled29IUGRpreterminfants,38preterminfantsbornappropriateforgestationalage
6、(AGA),and20healthyfull-terminfants.Peripheralbloodwassampledduringthefirst24hoursoflife,andagainatacorrectedageof38weeksofthepreterminfants.Tlymphocytesubsetswereanalyzedbyflowcytometry,andabsolutecountsofleukocytes,totallymphocytes,andTlymphocyteswerede
7、terminedwithanautomated++hematologyanalyzer.ResultsWithinthefirst24hoursoflife,percentagesofCD3andCD4werelowerinIUGR+++preterminfantsthaninAGApreterminfantsandfull-terminfants(P<0.05),percentagesofCD8andCD4/CD8ratio++++werelowerinIUGRpreterminfantsthanin
8、full-terminfants(P<0.05),andpercentagesofCD3,CD4andCD4/CD8ratiowerelowerinAGApreterminfantsthaninfull-terminfants(P<0.05).Moreover,theabsolutecountsoftotallymphocyteswerelowerinIUGRpreterminfantsthaninfull-terminfants(P<0.