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1、Canoco软件操作的简单教程步骤：1．打开excel文档，如下图所示，包含环境变量（12个）和生物因子（15种C源利用性）。共是8个处理，每个处理三个重复，总共是24个土壤样品。Potential:potentiallymineralizableorganicCpools;SOC,solubleorganicCextractedby2MKCl;SON,solubleorganicNextractedby2MKCl;soilmoisturecontent;TC,TotalC;TN,TotalN;WHC,waterholdingcapacity;MBC,microb
2、ialbiomassC;MBN,MicrobialbiomassN.The15Csourceswerechosentocoverarangeoflowmolecularweightcompounds(includingcarbohydrates,aminoacidsandcarboxylicacids)thatarecommonlyinrootexudates(Campbelletal.,2008).Thenumbersfrom1to16correspondtorepresentL-alanine,L-arginine,citricacid,D-galactose
3、,aminobutyricacid,malicacid,oxalicacid,3,4-OHbenzoicacid,L-arabinose,L-cysteine,D-fructose,D-glucose,L-lysine,N-acetylglucosamine,D-trehalose,water,respectively.6 选中区域（包括前面的数字），点击复制，然后打开canoco软件中WcanoIMP，如下图所示。然后选中saveincondensedformat，然后点击save。分别保存文件为environ.dta(dta是canoco默认的样式)和spec
4、ies.dta.文件形式如下图。62.打开canoco软件—newproject，得到下面的界面。63．点下一步后，有三个brown窗口，前2个分别选择environ.dta和species.dta.第三个选择软件自带合适的处理方法（如CCA和RDA等）。本文是选用RDA分析。4．其他还有一些窗口，但是问题不大，这里就不多说了。直接进入下一步，点击analyze，如下图所示。完成后点击canodraw。65．点击create窗口，如下图所示。得到最后的结果。结果的解释：Therelationshipsbetweenenvironmentalfactorsandmi
5、crobialmetabolicprofileswereasshownabove.Plotscanbeinterpretedquantitativelyusingthelengthofthearrowtoindicatehowmuchvariancewasexplainedbythatfactor;thedirectionofthearrowsforindividualenvironmentalfactorindicatinganincreasingweightingofthatfactor.Thesoilsamplearrowspointinginapproxi
6、matelythesamedirectionastheenvironmentalarrowsindicateahighpositivecorrelation(thelongerthesoilsample6arrow,thestrongertherelationship;Kennedyetal.,2004).Amongallenvironmentalfactors,C0exhibitedthegreatestinfluenceonthesoilsamples(P<0.01)followedbypHandsoilmoistureandSONcontent.Whatis
7、drivingchanges?PreviousstudieshavesuggestedthatRDAwasasuitablemethodforquantifytherelationshipbetweenenvironmentalfactorsandchangesinmicrobialcommunityofsoils(Kennedyetal.,2004;Macdonaldetal.,2009).Inthisstudy,wefoundthatclassifiedmicrobialmetabolicprofilesbasedoncovercroptreatmentsan
8、dquan
