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1、TheorApplGenet(2007)114:595–608DOI10.1007/s00122-006-0460-6ORIGINALPAPERMappingandcharacterizationofFLChomologsandQTLanalysisofXoweringtimeinBrassicaoleraceaK.Okazaki·K.Sakamoto·R.Kikuchi·A.Saito·E.Togashi·Y.Kuginuki·S.Matsumoto·M.HiraiReceived:27September2006/Accepted:6November2006/Publish
2、edonline:29November2006©Springer-Verlag2006AbstractTheFLCgeneproductisaninhibitorof(cabbage;vernalizationtype),whichcovered540cM,9XoweringinArabidopsis.FLChomologsinBrassicamajorlinkagegroups.Sixquantitativetraitloci(QTL)speciesarethoughttocontrolvernalization.WeclonedcontrollingXoweringtim
3、eweredetected.BoFLC1,fourFLChomologs(BoFLCs)fromBrassicaoleracea.BoFLC3andBoFLC5werenotlinkedtotheQTLsThreeofthese,BoFLC1,BoFLC3andBoFLC5,havecontrollingXoweringtime.However,thelargestQTLbeenpreviouslycharacterized.ThefourthnoveleVectwaslocatedintheregionwhereBoFLC2wassequencedisplayed98%se
4、quencehomologytothemapped.GenotypingofF2plantsattheBoFLC2locuspreviouslyidentiWedgeneBoFLC4,butalsoshowedshowedthatmostoftheearlyXoweringplantswere91%homologytoBrFLC2fromBrassicarapa.Phylo-homozygotesofBoFLC-GC,whereasmostofthelate-geneticanalysisshowedthatthisclonebelongstotheandnon-Xoweri
5、ngplantswerehomozygotesofFLC2clade.Therefore,wedesignatedthisgeneBoFLC-Rei.TheBoFLC2homologspresentinplantsBoFLC2.BasedonthesegregationofRFLP,SRAP,ofthenon-vernalizationtypewerenon-functional,dueCAPS,SSRandAFLPloci,adetailedlinkagemapoftoaframeshiftinexon4.Moreover,duplicationsandB.oleracea
6、wasconstructedintheF2progenyobtaineddeletionsofBoFLC2weredetectedinbroccoliandafromacrossofB.oleraceacv.GreenComet(broccoli;rapidcyclingline,respectively.Theseresultssuggestnon-vernalizationtype)andB.oleraceacv.ReihothatBoFLC2contributestothecontrolofXoweringtimeinB.oleracea.CommunicatedbyH
7、.C.Becker.IntroductionK.Okazaki(&)·R.Kikuchi·E.TogashiFacultyofAgriculture,NiigataUniversity,Niigata950-2181,JapanBrassicaoleraceaL.isoneofthemostimportantcrope-mail:okazaki@agr.niigata-u.ac.jpspeciesandincludesmanyvegetablessuchascabbage,broccoli,Brusse
