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1、作物学报ACTAAGRONOMICASINICA2010,36(2):347−353http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2010.00347不同小豆品种(系)叶片衰老与活性氧代谢*宋慧冯佰利高小丽高金锋王鹏科柴岩张盼盼西北农林科技大学农学院,陕西杨凌712100摘要:以高产小豆品种(系)2000-75、冀红9218和低产品种(系)红宝1号、湾选1号
2、为材料,研究小豆开花至成熟期主茎开花节叶片的叶绿素含量、可溶性蛋白含量、酶促防御系统保护性酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和丙二醛(MDA)积累量的变化规律。结果表明,小豆开花后,随着衰老进程的推进,主茎开花节位叶片自下向上依次衰老,其叶绿素、可溶性蛋白含量、SOD、CAT活性均呈下降趋势,POD活性、MDA积累量持续上升。与低产品种(系)相比,高产品种(系)主茎开花节位叶片衰老缓慢,生育后期仍保持较高的叶绿素、可溶性蛋白含量和SOD、CAT活性,籽粒产量高。相关分析表明,小豆产量与生育后
3、期各节位叶片的叶绿素、可溶性蛋白含量、SOD、CAT活性呈极显著正相关,POD活性和MDA含量与产量呈现显著负相关。研究表明,小豆生育后期,有效控制和延迟叶片衰老,对籽粒产量形成具有重要作用。关键词:小豆;叶片衰老;叶绿素含量;活性氧代谢LeafSenescenceandReactiveOxygenMetabolisminDifferentAdzukiBeanCultivars(Lines)*SONGHui,FENGBai-Li,GAOXiao-Li,GAOJin-Feng,WANGPeng-Ke,CHAIYan,andZHA
4、NGPan-PanCollegeofAgronomy,NorthwestA&FUniversity,Yangling712100,ChinaAbstract:Toexaminetherelationofleafsenescenceanditsreactiveoxygenmetabolitesfromdifferentnodesofvariousadzukibeancultivars,toexploretheagingmechanismsofadzukibeanleaf,tofindouttheintrinsicyield-fo
5、rmingmechanismsandtopro-videatheoreticalbasisforhigh-yieldbreedingandproductionofadzukibeans.Weconductedafieldexperiment,twohigh-yieldingcultivars,2000-75andJihong9218,andtwolow-yieldingcultivars,Hongbao1andWanxuan1,alladoptedinthesummerplantingecologicalregionofChi
6、na,weregrownin2008andtheirleafphysiologicalindices,suchaschlorophyllcontent,soluble-proteincontent,SOD,CATandPODactivitiesandMDAaccumulations,weremeasuredfromfloweringtoripeningstages.Theresultsshowedthatafterfloweringofthecultivars,theirfunctionalleavesagedgraduall
7、yfrombottomnodestotopnodes,andshowedde-creasedchlorophyllandsoluble-proteincontents,decreasedSODandCATactivitiesandincreasedPODactivitiesandMDAaccu-mulationsintheproceedofsenescence,forwhichthereweresignificantdifferencesamongcultivars.Comparedwiththelow-yieldingvar
8、ieties,theleavesofthehigh-yieldingcultivarsagedslower,showingrelativelyhighercontentsofchlorophyllandsoluble-protein,higheractivitiesofSOD
