斑马鱼下颌损伤后的再生组织的超微结构观察及神经递质分析

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1、HansJournalofAgriculturalSciences农业科学,2014,4,73-80PublishedOnlineAugust2014inHans.http://www.hanspub.org/journal/hjashttp://dx.doi.org/10.12677/hjas.2014.44012UltrastructuralObservationandNeurotransmitterAnalysisofTissueRegenerationafterAmputationofZebrafishLowerJawHao

2、Wu1,LiLi2,XianruiLi3,JiyuWang3,DanqunChen4,JizhouYan31KeyLaboratoryofFreshwaterFisheryGermplasmResources,MinistryofAgriculture,CollegeofFisheriesandLifeSciences,ShanghaiOceanUniversity,Shanghai2GuangxiFangchenggangSubversionBureauofQualityandTechnique,Fangchenggang3Dep

3、artmentofBiology,CollegeofFisheriesandLifeSciences,ShanghaiOceanUniversity,Shanghai4PutouDistrictFisheriesManagementCenter,ZhoushanCityEmail:hwu@shou.edu.cnththrdReceived:May27,2014;revised:Jun.16,2014;accepted:Jun.23,2014Copyright©2014byauthorsandHansPublishersInc.Thi

4、sworkislicensedundertheCreativeCommonsAttributionInternationalLicense(CCBY).http://creativecommons.org/licenses/by/4.0/AbstractZebrafishisanidealmodelforstudyingorganregeneration,particularlyincomplicatednervetissuestructureslikelowerjawandcaudalfin.Inthisstudy,wedynam

5、icallyobserveultrastruc-turalchangesofwoundtissuesandanalyzefourneurotransmittercontentsduringtheearlystageoflowerjawregenerationafterremovalofonethirdofmandibleandassociatesofttissues.Twohourspost-amputation(2hpa),contractionoccursaroundtheedgesofthewoundcausingthewou

6、ndsizetobecomesmaller.Incomparisonwithnon-amputation,thelevelofdopamine,γ-Aminobutyricacid(GABA),glutamicacidandglycineincreaseinthewoundtissues.Thecontentoffourneurotransmittersreachestothepeakwhenepidermisreconstitutioniscompleteat2dpa.At5dpa,themissingstructureislar

7、gelyrecoveredwhilethe4neurotransmittersretainthehighlevelorslightlyreduce.Theseresultssuggestthatneurotransmittersplayimportantrolesinin-troducinglowerjawregeneration.KeywordsZebrafish,LowerJaw,Regeneration,Neurotransmitter73斑马鱼下颌损伤后的再生组织的超微结构观察及神经递质分析斑马鱼下颌损伤后的再生组织的超微结

8、构观察及神经递质分析123343吴昊，李利，李先瑞，王箕雨，陈丹群，严继舟1上海海洋大学水产与生命学院农业部淡水水产种质资源重点实验室，上海2广西防城港市质量技术监督局，防城港3上海海洋大学水产与生命学院生物系4浙江舟山市普陀区渔业经