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1、Symmetry®Symmetry®•SymmetryShield™•Symmetry300™•SymmetryPrep™SolutionsforCommonHPLCColumnProblems••Symmetry®••Symmetry®••SymmetryShield™••SymmetryShield™Symmetry®••Symmetry300™•••SymmetryPrep™•Symmetry®—Symmetry®C3.5,5µm100Å19%18C3.5,5µm100Å12%8SymmetryPrep™C7µm100Å19%18C
2、7µm100Å12%8SymmetryShield™RP3.5,5,7µm100Å15%8RP3.5,5,7µm100Å17%18Symmetry300™C3.5,5µm300Å8.5%18C3.5,5µm300Å2.8%4Symmetry®HPLCSymmetry®C,WatersAlliance®HT2795184.6x150mm,5µmWaters996PDAdetectorA5BCpH3.75;100mMSymmetry®NHCOOH41.4mL/min30%A;60%B;10%CRSD5.0µL1.0.7%30°CUV@233n
3、m2.0.8%3.0.6%4.0.7%12Symmetry®3HPLC4Symmetry®136(1998)142(1998)HPLC149(1999)HPLC151(2000)Symmetry®156(2001)0.001.503.004.506.007.509.0010.5012.0013.5015.00Symmetry®WaterSymmetry®221Symmetry®C18Symmetry®3.093.31Symmetry®C182.753.75X2.6YSymmetry®C181.522.533.54Cµmol/m218aa2
4、®(a)Symmetry1.81.6Na-1K1.4R=()()()24aK+121.2Symmetry®C181R=N=0.8a=1.522.533.54k=Cµmol/m218*4.142.86Symmetry®C18X4.700.81Y5.370*—10,0001Symmetry®C3.9mmx150mm,5µm83™®SentrySymmetry10,0003.9mmx20mm79:20:141.0mL/minute5210µL625°CUV@2541.Symmetry®2.3.4.5.6.10,0000246810Symmetr
5、y®10,000Sentry™2pHpHpH51.2.4®SymmetrypH2–8C183USPpH2Symmetry®C1812345678pHSymmetry®pHSymmetry®C3.5µm2.1mmx10mmSymmetry®C3.5µm2.1mmx20mm18181250%®SymmetryC3.5µm2.1mmx30mm183Symmetry®C3.5µm2.1mmx50mm+184+A0.1%TFAB0.1%TFA1.0mL/min+50%*%A%B0.001000+4.0040601µL30.0°CUV@254nm*1
6、,230.000.501.001.502.002.503.003.504.004.503SymmetryShield™SymmetryShield™HPLCWaterspH7SymmetryShield™RP1.218™SymmetryShieldRP18L1.5USPHP1.66HE1.75ODO1.78SiDA1.9I1.95CH3CH3P2.39E3.601234USPa:SymmetryShield™RP™18a.SymmetryShield™RP3.9x150mmSymmetryShield18b.C121865%35%20mM
7、4KHPO/KHPO@pH72424™1.0mL/minuteSymmetryShieldUV@254nm23°C3+pH1.2.3.USP=1.254.DipropylphthalateAU+CC65.1886.77.pH2-8SymmetryShield™USP1.2051015202530SymmetryShield™b:C181234AU5USP=1.9760510152025304SymmetryShield™SymmetryShield™6,748CC1118810C89™3.9x150mmSymmetryShield1512
8、2305101520253035404550™4SymmetryShield7811SymmetryShield™RP6108C893.9x150mm125123051015202530354
