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1、NEURALREGENERATIONRESEARCHVolume7,Issue7,March2012www.nrronline.orgCitethisarticleas:NeuralRegenRes.2012;7(7):485-491.Dorsalrootganglionprogenitorsdifferentiatetogamma-aminobutyricacid-andcholineacetyltransferase-positiveneurons*☆○1,21,21,2111,2LingliYu,YindiDin
2、g,AmbreSpencer,JiMa,RuishengLu,BrianB.Rudkin,1ChonggangYuan1LaboratoryofMolecularandCellularNeurophysiology,SchoolofLifeScience,EastChinaNormalUniversity,Shanghai200062,China2Differentiation&CellCycleGroup,LaboratoryofMolecularandCellularScience,UMR5239CNRS/ENSL
3、yon/UniversityofLyon1,EcoleNormaleSupérieuredeLyon,IFR128”BiosciencesLyon-Gerland”Lyon,69007,FranceAbstractThisstudyexaminedtheisolationanddifferentiationofdorsalrootganglionprogenitorcellsforLingliYu☆,Studyingfordoctorate,Laboratoryoftherapeuticuseinneurodegene
4、rativediseases.RatembryonicdorsalrootgangliaprogenitorswereMolecularandCellularisolatedandpurifiedusingthedifferentialadhesionmethodcombinedwithcytosinearabinosideNeurophysiology,SchoolofLifeScience,EastChinatreatment.Aftercultureinserum-freemediumsupplementedwi
5、thB27,basicfibroblastgrowthfactorNormalUniversity,Shanghaiandepidermalgrowthfactor,thesecellsremainedviableandsurvivedformorethan18monthsin200062,China;vitro.Mostcellsdifferentiatedtoneuronsthatwereimmunoreactiveforgamma-aminobutyricacidDifferentiation&CellCycle
6、Group,Laboratoryofandcholineacetyltransferaseasdetectedbyimmunohistochemicalstaining.Inaddition,nerveMolecularandCellulargrowthfactorandneurotrophictyrosinekinasereceptorexpressionwerealsoobservedindorsalScience,UMR5239CNRS/ENSLyon/Universityrootganglionprogenit
7、orsanddifferentiatedcells.K252a,aninhibitorthatblocksnervegrowthofLyon1,EcoleNormalefactor-inducedsignaling,inhibitedcellsurvival,suggestingthepossibleexistenceofanervegrowthSupérieuredeLyon,IFR128”Biosciencesfactorautocrineloopintheseproliferatingcells.Lyon-Ger
8、land”Lyon,69007,KeyWords:dorsalrootganglion;neuralprogenitor;differentiationcharacterization;nervegrowthFrancefactor;tyrosinekinasereceptortype1LingliYuandYindiDingAb
