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1、Real-TimePCR新技术新应用基因组-DNA水平转录、调控-RNA水平Protein水平GenomicSequenceSNPsCNVsMethylation2主要内容•GenotypingSolution•荧光定量PCR其他应用−microRNA研究的整体解决方案−基因拷贝数CNV−肿瘤稀有突变检测−TaqMan蛋白表达分析试剂盒3SingleNucleotidePolymorphisms(SNPs)单一碱基序列多态性ASingleNucleotidePolymorphism(SNP)isachangein
2、thenucleotidepresentatasinglebaseinaknownDNAsequence.ATGCATGCATGGCATGCATGCATGCATGCATAACATGCATGCThisG/AisaSNP4GTMarketPositioningMarkers•全基因组关联性分析500,000•发现新SNP•发现新遗传多态发现发现•CandidateRegion/Gene100,000•Finemapping•Diseaseassociation10,000•ADME/Tox验证验证•Traitsele
3、ctionandbreeding,QC1,000•Largesample•ADME/Toxcandidategene/•CandidateGenemarkerscreening100Commercialization/Commercialization/CandidateGene/CandidateGene/ScreeningScreeningClinical&DxClinical&DxSamples1001,00010,000100,0005
4、LifeTechnologiesProprietary&Confid
5、ential
6、11/10/2010AB’sCompleteGenotypingSolutionsMarkers•WholeGenomeAssociation500,000•SNPdiscoveryDiscoveryDiscovery•GeneticVariationdiscovery•CandidateRegion/Gene100,000•Finemapping•Diseaseassociation10,000•ADME/ToxValidationValidation•Candidategene/markersc
7、reening1,000•ADME/Tox•CandidateGene100Commercialization/Commercialization/CandidateGene/CandidateGene/ScreeningScreeningClinical&DxClinical&DxSamples1001,00010,000100,0006
8、LifeTechnologiesProprietary&Confidential
9、11/10/2010SNP位点的发现新一代超高通量测序技术TechnologyhasCost
10、perAcceleratedHumanGenomeG3$M$100,000,000100,000.00G210,000.00Single-moleculesequencing1,000.00G1100.00$100,000ABISOLiDMassivelyparallel10.00sequencingbysynthesisG0ABI3730xl1.00Dye-labeledterminators&$10,000capillaryelectrophoresis0.100.01SequencinggelsTime‘9
11、0‘95‘96‘01‘02‘03‘04‘06‘07‘082-3yrs7AbreathtakingpaceofinnovationResequencing：医学测序/比较基因序列分析•AmethodthatcomparessequencesobtainedfromoneormultipleDNAsamplestoaknownreferencesequence−ToidentifyvariationsinDNAsequence−Tocorrelatechangeswithimportantscientificques
12、tionsDenovo:•1base/peakResequencing:•Mixedbases/peak8AB’sCompleteGenotypingSolutionsMarkers•WholeGenomeAssociation500,000•SNPdiscoveryDiscoveryDiscovery•GeneticVariationdiscovery•Candidat
