幻灯片 1 - University of Lethbr

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1、Comparingmorphologicalandphysiologicalcharacteristicsbetweenwildtypeandfkd1ArabidopsisChenLiu2012-04-11Introduction•Arabidopsisisafacultativelong-dayplantwhichflowersearlierinlongdaycondition.•Wildtype(wt)Arabidopsishasatypicalcomplexandclosedleafveinpattern,whilethefkd1mutant(lossoffun

2、ctionofFORKED1gene)hasadefectiveveinpatternwithnon-meetingdistalveins.Differentvenationpatternsofwtandfkd1Arabidopsis•Cotyledonswtfkd1•FirstleavesScalebar:1mmwtfkd1Differentrosetteleafshapeofwtandfkd1Arabidopsiswtfkd1Scalebar:5cm•Distalmeetingofvenationisproposedtobeadvantageous:provid

3、esredundancyintransportationroutes,incaseofinjuryorblockageofavascularbundle;increasesmechanicalstabilityandsupporttoleaf,particularlyalongleafmargin.•Hypotheses:Longerdaylengthcouldpromoteearlierfloweringandpreventvegetativegrowthofbothwtandfkd1Arabidopsis.Assumingthedefectiveveinpa

4、tternoffkd1makesitlessefficientinwateruse,resultsindecreasedgrowthrateorphotosyntheticcapacity;otherwise,changesinchlorophyllcontentorstomatalconductancecouldcompensateforreducedwaterdeliveryinfkd1.Materialandmethods•Plantmaterial:Wildtypeseeds-ArabidopsisBiologicalResourceCenterfkd1mut

5、antseeds-previouslyEMS-mutagenizedandscreened•Growthconditions:Allseedsweregerminatedinsoil,oneplantperpot(36cm2);growthchambergrownforfourweeks;wateredonceaweekwithdeionizedwater.Soilmixtype:three-quarterspottingsoil,one-quartervermiculiteLightintensity:130μmols-1m-2Relativehumidit

6、y:60%Differentdaylengths:24-hourdaylengthat22°C16-hourdaylengthat22°C,8-hournightlengthat18°C•Formorphologicalcharacteristicsanalysis,plantsweregrownundertwodifferentdaylengths.RosettediameterWholeplantbiomassdryweight•Forphysiologicalcharacteristicsanalysis,plantsweregrownunder16-ho

7、urdaylength.COassimilationrate&transpirationrate2Water-useefficiency(WUE)Totalleafconductance:g&gwcChlorophyllcontentStomatalsize&density:abaxial&adaxialsurfacesResultswtfkd124-hourdaylength16-hourdaylength24-hourdaylength16-hourdaylengthFigure1.Morphologicalcharactersof4-