幻灯片 1 - University of Lethbridge

《幻灯片 1 - University of Lethbridge》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、Comparingmorphologicalandphysiologicalcharacteristicsbetweenwildtypeandfkd1ArabidopsisChenLiu2012-04-11Introduction•Arabidopsisisafacultativelong-dayplantwhichflowersearlierinlongdaycondition.•Wildtype(wt)Arabidopsishasatypicalcomplexandclosedleafveinpattern,whilethefkd1mutan

2、t(lossoffunctionofFORKED1gene)hasadefectiveveinpatternwithnon-meetingdistalveins.Differentvenationpatternsofwtandfkd1Arabidopsis•Cotyledonswtfkd1•FirstleavesScalebar:1mmwtfkd1Differentrosetteleafshapeofwtandfkd1Arabidopsiswtfkd1Scalebar:5cm•Distalmeetingofvenationisproposedto

3、beadvantageous:providesredundancyintransportationroutes,incaseofinjuryorblockageofavascularbundle;increasesmechanicalstabilityandsupporttoleaf,particularlyalongleafmargin.•Hypotheses:Longerdaylengthcouldpromoteearlierfloweringandpreventvegetativegrowthofbothwtandfkd1Arabid

4、opsis.Assumingthedefectiveveinpatternoffkd1makesitlessefficientinwateruse,resultsindecreasedgrowthrateorphotosyntheticcapacity;otherwise,changesinchlorophyllcontentorstomatalconductancecouldcompensateforreducedwaterdeliveryinfkd1.Materialandmethods•Plantmaterial:Wildtypeseed

5、s-ArabidopsisBiologicalResourceCenterfkd1mutantseeds-previouslyEMS-mutagenizedandscreened•Growthconditions:Allseedsweregerminatedinsoil,oneplantperpot(36cm2);growthchambergrownforfourweeks;wateredonceaweekwithdeionizedwater.Soilmixtype:three-quarterspottingsoil,one-quarterv

6、ermiculiteLightintensity:130μmols-1m-2Relativehumidity:60%Differentdaylengths:24-hourdaylengthat22°C16-hourdaylengthat22°C,8-hournightlengthat18°C•Formorphologicalcharacteristicsanalysis,plantsweregrownundertwodifferentdaylengths.RosettediameterWholeplantbiomassdryweight

7、•Forphysiologicalcharacteristicsanalysis,plantsweregrownunder16-hourdaylength.COassimilationrate&transpirationrate2Water-useefficiency(WUE)Totalleafconductance:g&gwcChlorophyllcontentStomatalsize&density:abaxial&adaxialsurfacesResultswtfkd124-hourdaylength16-hourdaylengt

8、h24-hourdaylength16-hourdaylengthFigure1.Morphologicalcharactersof4-