土壤中几种芽孢杆菌的研究

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1、土壤中几种芽孢杆菌的研究TheResearchoftheBacillusinSoil李小凡山东大学生命科学学院2002年12月25日摘要本实验中，通过对土壤溶液的浓度梯度分离纯化和选择性培养基的培养，取得了土壤中有代表性菌株，并对其中芽孢杆菌属(Bacillus)的几株做了比较详细的，形态学的分析和归类，包括巨大芽孢杆菌(Bacillusmegaterium)，胶质芽孢杆菌(Bacillusmucilaginosus)，枯草芽孢杆菌(Bacillussubtilis)，多粘性芽孢杆菌(Bacilluspolymyxa)等等。其后，还对某些

2、菌株作了生理生化实验，通过实验进一步分析菌株的生理特征，并验证菌株的分类。AbstractTogetacommonideaforwhatkindsofbacteriathatsoilcontains,especiallyforspeciesofBacillus,wedidconcentrationgradientofsoilandmadeinoculationon2selectiveculturemedia.Byresearchonmorphologicobservationandanalysis,severalstrainswereid

3、entified,i.e.Bacillusmegaterium,Bacillusmucilaginosus,Bacillussubtilis,Bacilluspolymyxa.Meanwhile,wedidphysiologicaltestoftwostrainsweselected,withthecomparisonof3othertypicalstrains:Escherichiacoli,Bacilluspyocyaneus,andStaphylococcusaureus.Theresultsofourphysio-logicalt

4、estvalidatedouranalysisandclassification.关键词土壤芽孢杆菌浓度梯度形态学生理生化keywordssoil,bacillus,concentrationgradient,morphologic,physiological正文目录1．概述......................................................................................................................................

5、.......12．详细过程.....................................................................................................................................22.1.2试管、平板及其他准备.........................................................................................22.1.3土壤溶液的制备.......

6、..............................................................................................32.1.4接种与培养.............................................................................................................32.1.5分离纯培养...............................................

7、..............................................................32.1.6观察结果.................................................................................................................32.2.1试剂的准备............................................................................

8、.................................32.2.2菌种的选择....................................................