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1、http://www.paper.edu.cnIntragastricAdministrationofEvodiamineReducesNPYandAGRPGeneExpressioninTheHypothalamusand1DecreasesFoodIntakeinRatsShiJinghong，YanJianqun*，LeiQi，ZhaoJunjie，ChenKe，YangDejun，ZhaoXiaolinDepartmentofPhysiologyandPathophysiology，KeyLa
2、boratoryofEnvironmentandGenesRelatedtoDiseases，MinistryofEducation，Xi’anJiaotongUniversitySchoolofMedicine，Xi’an（710061）E-mail：jqyan@mail.xjtu.edu.cnAbstractEvodiamine(Evo),analkaloidalcomponentextractedfromthefruitofEvodiaefructus(EvodiarutaecarpaBenth
3、.,Rutaceae),decreasesthebodyweightofratsthroughapoorlydefinedmechanism.Thehypothalamusisresponsibleforcontrollingfoodintakeandenergyexpenditure.WepostulatethatEvomediatesthisactivitybymodulatingfeeding-relatedpeptidesinthehypothalamus.Weinvestigatedthee
4、ffectsofEvoonfoodintake,bodyweightandthemRNAexpressionofhypothalamicNPY,AGRP,Pmch,POMC,CART,andMC4R,inmalerats.Evo(40mg/kgor4mg/kg)wasintragastricallyadministratedfor25days,andthefoodintakeandbodyweightofratswererecordeddaily.Bloodsampleswerecollectedfo
5、rleptinradioimmunoassay(RIA).Real-TimePCRwasusedtoanalyzethemRNAexpression.OurresultsshowthatintragastricadministrationofEvo(40mg/kg)inhibitsfoodintake,decreasesbodyweightandreducesthemRNAexpressionofNPYandAGRPinthehypothalamus,whileitincreasesthecircul
6、atinglevelofleptin.OurresultsindicatethatEvodecreasesfoodintake,andthereforebodyweight,partlybydown-regulatingNPYandAGRPmRNAexpressioninthehypothalamus.Keywords：evodiamine，leptin，NPY，AGRP，foodintake1.IntroductionEvodiamine(Evo)isamajoralkaloidalcomponen
7、tofthedried,unripefruitofEvodiarutaecarpaBentham(Rutaceae),andisanagonistofvanilloidreceptor1(VR1)andhasnoperceivabletaste(includingapepperyhottaste).PreviousinvestigationshaveindicatedthatEvohasanumberofpharmacologicalactionssuchasdecreasingbodyweight,
8、regulatingbodytemperature,anti-tumoractivity,painrelief,heartprotection,decreasingbloodpressureandinducingendocrineeffects[1-6].PreviousstudydemonstratedthatEvoexhibitsCapsaicin-likeanti-obeseactionswhichmaystimulateVR1intheprima
