不同月经周期进行未成熟卵体外成熟培养 体外受精-胚胎移植的探讨

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1、不同月经周期进行未成熟卵体外成熟培养/体外受精-胚胎移植的探讨【关键词】月经【摘要】目的比较在不同月经周期进行未成熟卵体外成熟培养/体外受精-胚胎移植的结果。方法分别在少量应用促性腺激素（Gn）周期（从月经第5天开始肌肉注射HMG150IU/d，4～5天后，当最大卵泡直径达10～12mm时肌肉注射HCG10000IU后36h取卵）和未刺激周期（于月经第10～12天肌肉注射HCG10000IU，36h后取卵）采集未成熟卵进行体外培养/体外受精和胚胎移植，将培养、受精结果及临床妊娠结果进行比较。结果共125例患者182个周期，少量应用Gn组55

2、例88个周期，未刺激组70例94个周期，2组在年龄、不孕年限和基础内分泌情况方面没有明显差异。在未刺激周期，卵子的体外成熟率明显低于少量应用Gn周组（分别为65.3%和72.7%，P<0.05），受精率、卵裂率和优质胚胎率2组没有显著差别（P>0.05）。在应用少量Gn组，共移植37个周期，获得1例临床妊娠，未刺激组移植75个周期，临床妊娠18例，妊娠率24.0%，胚胎着床率6.0%。结论未刺激周期更适合进行未成熟卵体外培养/体外受精-胚胎移植，临床妊娠率接近传统IVF/ICSI，但胚胎着床率偏低。关键词未成熟体外成熟妊娠Invitroma

3、turation/fertilizationofoocytesandembryotransferinunstimulatedcyclesandmildstimulatedcyclesinpolycysticovariansyndromepatientsLiYuan，ChenZijiang，ZhaoLixin，etal.ReproductiveMedicalCenterofShandongUniversityandShandongProvincialHosptial，Jinan，250021.【Abstract】ObjectiveCompar

4、edtheresultsofinvitromaturationandfertilizationofoocytesretrievedinunˉstimulatedcyclesandmildstimulatedcycles.MethodsInmildstimulatedcycles，thepatientsweregiven150IU/dHMGfor4to5daysuntilthepromotingfolliclediametereached10to12mm，then36thafter10000IUHCGwasgivˉen，theoocytesr

5、etrievalwouldbeperformed.Inunstimulatedcycles，thepatientswouldbegivenHCGonday10to12basedontheultrasoundobservationthatnofollicleslargerthan8mm.Theimmatureoocyteswereculturedandfertilˉized，theresultedembryosweretransferredbacktothefemales’uterus.Comparetheresultsofinvitroma

6、turationandfertilization，andtheclinicalpregnantresultswerealsocompared.ResultsOnehundredeighty-twoinvitromaturaˉtioncycleswereperformedon125patients，88wereprimedwithlittleamountofGnandother94cycleswwerenot.Theage，durationofinfertilityandbasalendocrinologywerehavenodifferen

7、cebetweenthesetwogroups.Innaturalcycels，thematurationratewassignificantlylowerthanGn-priminggroup（65.3%and72.7%，P<0.05，buttherewasnodifferencebetweentheminfertilizationrate，cleavagerateandhighqualityembryorate（P>0.05）.InGn-primingcycles，embryosweretransferredbacktouterusin

8、37patients，butonly1gotpregnant.Inunstimulatedcycles，75hadembryostransferredand18werepregn