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1、中国神经科学杂志2003March,19(2)ChinJNeurosci113·研究报告·白腰文鸟原纹状体栎核发声通路与SP2免疫组化的定位12222张信文,曾少举,李佳,马骏,左明雪(1.海南师范学院生物学系,海南海口571158;2.北京师范大学生命科学学院,北京100875)[摘要] 目的:对白腰文鸟端脑原纹状体栎核(RA)的神经联系和SP2免疫阳性神经细胞或纤维在发声通路中的定位进行研究。方法:采用生物素结合的葡聚糖胺(BDA)双向示踪方法和P物质(substanceP,SP)免疫组织化学技术。将BDA微量注入RA内,经ABC反应。结果:①RA接受发声控
2、制的高位中枢HVC和新纹状体前部巨细胞核(MAN)外侧部的传入投射,由RA发出的神经纤维投射到中脑背内侧核(DM)和延髓舌下神经核气管鸣管部(nXIIts),由此组成发声控制通路。②SP2免疫阳性神经细胞大量分布在端脑的HVC,MAN,RA内。SP2免疫阳性神经纤维或末梢密集分布在发声运动中枢中脑的DM、延髓的nXIIts、舌下神经,以及中脑背外侧核壳区(MLdshell)、中脑丘间核(ICo)和丘脑卵圆核壳(OVshell)内。结论:由于SP广泛分布在发声控制中枢内,提示SP可能在发声控制方面具有重要的生理功能。[关键词]白腰文鸟;原纹状体栎核;发声控制通路;
3、P物质;免疫组织化学Thevocalcontrolpathwayofrobustarchistriatumandimmunohistoche2micallocalizationofsubstancePinLonchurastriata12222ZHANGXin2wen,ZENGShao2ju,LIJia,MAJun,ZUOMing2xue(1.DeparmentofBiology,HainanNormalCollege,Haikou571158;2.CollegeofLifeScience,BeijingNor2malUniversity,Beijing1008
4、75,China)[Abstract]Objective:Toinvestigatetheneuronalconnectionsofrobustarchistriatum(RA)andtodeterminedistribu2tionofsubstancePinthevocalcontrolpathwayofLonchurastriata.Methods:Aneuraltracerbiotionylateddextranamine(BDA)method,andimmunohistochemicalmethodwereused.Results:①Afterinjec
5、tingBDAintoRA,labelledneuronswereobservedinhighvocalcontrolcenter(HVc),andthelateralnucleusmagnocellularoftheanteriorneostria2tum(LMAN).Labelledfibersandterminalswereobservedinnucleusdorsalismedialis(DM)andthenucleushypoglossi,parstracheosyringealis(nXIIts).②Alargenumberofneuronswith
6、substancePwereobservedinHVc,MANandRA.ThesubstancePlabeledfibersandterminalswereobservedinDM,nXIIts,thenervonshypoglossi(NXII),theshellofnucleusmesencephalicuslateralis,parsdorsalis(MLdshell),thenucleusintercollicularis(ICo)andthethalamicnu2cleus,ovoidalisshell(OVshell).Conclusions:RA
7、receivesprojectionsfromHVcandlateralMAN.TheRAprojectstoDMandnXIIts.SubstancePwaswidelydistributedinthevocalcontrolcentres,thereforeitmaybeinvolvedinimpor2tantphysiogicalfunctions.[Keywords]Lonchurastriata;robustarchistriatum;vocalcontrolpathway;substanceP;immunohistochemicalXmethod[文
8、章编号]10082087
