分子印迹聚合物的制备及在手性识别和手性拆分上的应用

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1、东南大学博士学位论文分子印迹聚合物的制备及在手性识别和手性拆分上的应用姓名：李萍申请学位级别：博士专业：生物医学工程指导教师：袁春伟20030501摘要AbstractThemolecularlyimprintedpolymer(MIP)，whichisbeingdevelopedinrecentyears，isakindoferosslinkedmaterialwithfixedcavitiesshapeandarrangedfunctionalgroups．Becauseofitspredeterminativeandspecificmolecularre

2、cognitionability,aswellastheeasilypreparedabilityandIlighstability,theMIPcanbeusedwidelyinbiotechnology,clinicalmedicine．environmentalmonitoring．foodindustriesandsoon．TheM口alsoexhibitspromisingapplicationinracemoidresolutionwithchromatography,solid-phaseextraction，sensors，mimicenzym

3、ecatalystsandmembraneseparation．Inthisdissertation,MIPsaresynthesizedwitIlchiralsubstrates．suchasderivativesofphenylalanine，naproxenandderivativesofmandelicacid，astemplatestoinvestigatethepreparationofMIPsandtheirchiralrecognitionandchiralresolution．1．Introduction：Theessentialtheori

4、es，preparationtechnologiesandapplicationdevelopmentsofMrPsa∞reviewed．2．MIPsalesynthesizedwithL—phenylalanineethylester'L—phenylalanine1-hexylester,￡一phenylalaninecyclohexylesterandL-phenylalaninedodecaxylester∞templatesrespectively．Then，effectsoftheratiooffunctionalmonomerstotemplat

5、emolecules，thepolymerizationtemperatureandstructuresoftemplatesonthechiralresolutionofMIPsarestudied．ThestructureofL—phenylalaninacyclohexylesterissimulatedtoexplainthegoodchiralseparationcapabilityofrelativeMIP_Thechromatographicconditions，such∞mobilephases，concentrationsofsamplesa

6、ndtemperatureofcolumn，areoptimized．3．M1Pswithchiraldrug(S)-naproxenasthetemplatearepreparedwithdifferentfunctionalmonomers，crosslinkersandtheircontents，polymerizationsolution．TheresolutionabilitiesofMIPschiralstationaryphasearecompared．ThecavitiesinMIPsandthemorphologiesofMIPsarecha

7、racterizedusingliquidnitrogenadsorptionandscanningelectronicmicroscopy．ThebindingcapacityoftherS)-naproxenM碑isinvestigatedthrou曲bindingexpemnants．Scatchardanalysesshowthattherearetwoclassesofbindingsiteswithhi曲orlOWaffinityinMIPs，Thecomplexesformedby(S)-naproxenandfunctionalmonoersa

8、crylamidearesimulat