六年制细菌各论教案-新

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1、IndividualMicrobiologystaphylococcusG+streptococcusCoccipneumococcusmeningococcusG-GonococcusAerobeG-------entericbacilliBacillicorynebacteriumdiphtheriaandG+M.tuberculosisfacultativeanaerobeSpirillarbacteria----V.choleraobligateanaerobespore-forminganaerobicbacterianon-spore-forminganaer

2、obicbacteriaContent1.biologicalcharacteristics2.pathogenicity------pathogenicfactor，phathogenesis3.immunity4.laboratorydiagnosis(bacteriologicalmethods)5.preventionandtreatmentPathogenicCocci（pyogeniccocci）G+cocci–Staphylococcus,Streptococcus,Pneumococcus.PurulentG-cocci–Meningococcus,Gon

3、ococcus.InfectionSection1.StaphylococcusI.Biologicalcharacteristics1.Morphology:Grampositivecocci,arrangedinirregular,grape–likeclusters,capsuleinvivo2.Culture:temperature:28~38℃(37℃);pH:4.5~9.8(7.4)colony:1~2mm,circular,smooth,shinysurface,variouspigmentsonbloodagar-----hemolysis(S.aureu

4、s)MajorpropertiesofthreespeciesofstaphylococciMainpropertyStaph.aureusStaph.EpidermidisStaphsaprophyticusPigmentationGoldenyellowWhiteCitrineCoagulase+__Hemolysin+__SPA+__Pathogenicity+++-/+_233.Typing:S.aureushavebeendifferentiatedintodifferentphagetypes(importantaspectintheinvestigation

5、ofainfectionsource).4.Antigenicstructure:(1)SPA(staphylococcalproteinA)i)cellwallproteinofS.aureasii)itcombinesnonspecificallywiththeFc-portionofhumanIgGiii)antiphagocytosisiv)damageplatelet;activateBcellv)coagglutination(rapiddiagnostictechnic)5.Resistance:resistanttodry;heat(80℃,30min);

6、salttolerant(10~15%NaCl)Sensitivetoantibioticsandsulfonamides,butdrug-resistanceareeasilyproducedII.Pathogenicity1.Pathogenicfactor1).Invasiveness(1)Surfacestructure:SPA---anti-phagocytosis,Lipoteichoicacid,LTA----adherence(2)Enzyme:Coagulase:Anenzymewhichcausescoagulationinplasmacontaini

7、ngananticoagulantsuchascitrate,coagulaseisfoundinallpathogenicstrainsofStaphylococci.Roles:toinhibitthephagocytosisofphagocytesanddamageofbacteriacidesubstancesinhumorbycoatingtheorganismswithfibrin.twotypesofcoagulase:(1).Freecoagulase----anextracellularenzymewhich