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1、保鲜与加工加工研究StorageandProcess2015,15(1):32-38芦荟提取液及其降解液制备凝胶的抑菌性研究黄艳1,21,231,2,*1,2,蔡锦红,黄初笑,白新鹏,贾延勇(1.海南大学食品学院,海南海口570228;2.生物活性物质与功能食品开发重点实验室,海南海口570228;3.海南金芦荟生物工程有限公司,海南海口570216)摘要:以芦荟提取液及其不同条件降解液制备的芦荟凝胶为研究对象,通过单因素试验和正交试验,研究不同添加剂对芦荟凝胶抑菌效果的影响,优化抑菌芦荟凝胶的添加剂配方。结果表明,制备的芦荟凝胶对大肠杆菌和金黄色葡萄球菌均具较好的抑菌效果,且
2、不同降解液制备的凝胶抑菌性不同,说明降解条件影响了芦荟液中的抑菌活性物质,故表现出不同的抑菌效应。本试验优化结果表明,抑制大肠杆菌的芦荟凝胶添加剂最佳配方为:尿囊素浓度3.0mg/mL,10%(V/V)三乙醇胺水溶液0.010mL/mL,20%(W/V)NaOH水溶液0.015mL/mL,卡松添加量为0.8μL/mL;抑制金黄色葡萄球菌的芦荟凝胶添加剂最佳配方为:尿囊素浓度3.6mg/mL,10%(V/V)三乙醇胺水溶液0.010mL/mL,20%(W/V)NaOH水溶液0.015mL/mL,卡松添加量为1.0μL/mL。关键词:芦荟;提取液;降解液;凝胶;抑菌效应TheAnt
3、ibacterialEffectsoftheGelPreparedbyAloeExtractandDegradationSolution1,21,231.2,*1,2HUANGYan,CAIJin-hong,HUANGChu-xiao,BAIXin-peng,JIAYan-yong(1.CollegeofFoodScience,HainanUniversity,Haikou570228,China;2.HaikouKeyLaboratoryforBioactiveSubstanceandExploitationofFunctionalFood,Haikou570228,Chin
4、a;3.HainanGoldenAloeBiologicalEngineeringCo.,Ltd.,Haikou570216,China)Abstract:Takingthealoegelwithaloeextractanditsdegradationunderdifferentdegradationconditionsastheresearchobjects,theeffectsofdifferentadditivesontheantibacterialeffectofthealoegelwerestudiedandtheadditiveformulationofantiba
5、cterialaloegelwasoptimizedbysinglefactorandorthogonaltests.Theresultsshowedthat,thepreparedaloegelhadgoodantibacterialeffectsonEscherichiacoliandStaphylococcusaureus,andtheantibacterialeffectofaloegelwhichpreparedbydegradationliquidwasdifferent.Thedegradationconditionsaffectedtheantimicrobia
6、lsubstancesinaloegel,sotheyshoweddifferentinhibitoryeffects.Theoptimizationresultshowedthat,thebestadditiveformulaofinhibitiononE.coliwasasfollows:allantoinconcentration3.0mg/mL,10%(V/V)triethanolamineaqueoussolution0.010mL/mL,20%(W/V)NaOHaqueoussolution0.015mL/mLandkathon0.8L/mL;thebestaddi
7、tiveformulaofinhibitiononS.aureuswasasfollows:allantoinconcentration3.6mg/mL,10%(V/V)triethanolamineaqueoussolution0.010mL/mL,20%(W/V)NaOHaqueoussolution0.015mL/mLandkathon1.0μL/mL.Keywords:aloe;extract;degradationsolution;gel;inhibitoryeffect基金项目: